Goat KPNB1 / Importin Beta Polyclonal Antibody | anti-KPNB1 antibody

Goat Polyclonal to Human KPNB1 / Importin Beta

Cat. Num. AAA242101

• Gene Names: KPNB1; IMB1; IPO1; IPOB; Impnb; NTF97
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Chicken, Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: KPNB1 / Importin Beta; Polyclonal Antibody; Goat Polyclonal to Human KPNB1 / Importin Beta; Anti-KPNB1 / Importin Beta Antibody (C-Terminus) IHC-plus; KPNB1; Importin 90; Importin Beta; Importin beta-1 subunit; IPO1; IPOB; NTF97; Impnb; Importin 1; Karyopherin subunit beta-1; Nuclear factor p97; PTAC97; IMB1; Importin subunit beta-1; Importin-90; Karyopherin (importin) beta 1; Human KPNB1; anti-KPNB1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Chicken, Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human KPNB1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 876

• Applicable Applications for anti-KPNB1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (1:32000, 0.03 - 0.1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RRSKTNKAKTLAT from the C-terminus of human KPNB1 (NP_002256.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: KPNB1 / Importin Beta antibody was raised against synthetic peptide C-RRSKTNKAKTLAT from the C-terminus of human KPNB1 (NP_002256.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%); Opossum, Chicken, Lizard, Xenopus (92%); Stickleback, Pufferfish, Zebrafish (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-KPNB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (0.03 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 19923142
• NCBI GeneID: 3837
• NCBI Accession #: NP_002256.2
• NCBI GenBank Nucleotide #: NM_002265.5
• UniProt Accession #: Q14974; Q14637; Q53XN2; Q96J27; B7ZAV6; D3DTT3
• Molecular Weight: 81,179 Da
• NCBI Official Full Name: importin subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: karyopherin (importin) beta 1
• NCBI Official Symbol: KPNB1
• NCBI Official Synonym Symbols: IMB1; IPO1; IPOB; Impnb; NTF97
• NCBI Protein Information: importin subunit beta-1; PTAC97; importin 1; importin 90; importin-90; nuclear factor p97; importin beta-1 subunit; karyopherin subunit beta-1; pore targeting complex 97 kDa subunit
• UniProt Protein Name: Importin subunit beta-1
• Protein Family: Importin
• UniProt Gene Name: KPNB1
• UniProt Synonym Gene Names: NTF97; PTAC97
• UniProt Entry Name: IMB1_HUMAN

NCBI Description

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family.

Protein type: Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; nuclear membrane; membrane; cytoplasm; nuclear pore; nuclear envelope; cytosol

Molecular Function: protein domain specific binding; protein binding; enzyme binding; zinc ion binding; nuclear localization sequence binding; Ran GTPase binding; protein transporter activity

Biological Process: ribosomal protein import into nucleus; viral reproduction; protein import into nucleus, translocation; protein import into nucleus; apoptosis; cytokine and chemokine mediated signaling pathway; DNA fragmentation during apoptosis; viral infectious cycle; NLS-bearing substrate import into nucleus; cell structure disassembly during apoptosis