Rabbit Kv1.6 Polyclonal Antibody | anti-KCNA6 antibody

Kv1.6 Antibody

Cat. Num. AAA9612282

• Gene Names: KCNA6; HBK2; KV1.6; PPP1R96
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Kv1.6; Polyclonal Antibody; Kv1.6 Antibody; anti-KCNA6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Kv1.6 Antibody detects endogenous levels of total Kv1.6.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 529

• Applicable Applications for anti-KCNA6 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Kv1.6, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hek293 , using Kv1.6 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-KCNA6 antibody

Function: Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient (PubMed:2347305, PubMed:14575698). The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:2347305, PubMed:14575698). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA6, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed:2347305).

Subcellular Location: Cell membrane>Multi-pass membrane protein.

Subunit Structure: Homotetramer and heterotetramer of potassium channel proteins (By similarity). Interacts with KCNAB1 and KCNAB2 (By similarity).

Similarity: The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 116434
• NCBI GeneID: 3742
• UniProt Accession #: P17658
• Molecular Weight: Observed Molecular Weight: (Observed)58kD.; Predicted Molecular Weight: (Calculated)59kDa.
• NCBI Official Full Name: Potassium voltage-gated channel subfamily A member 6
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily A member 6
• NCBI Official Symbol: KCNA6
• NCBI Official Synonym Symbols: HBK2; KV1.6; PPP1R96
• NCBI Protein Information: potassium voltage-gated channel subfamily A member 6
• UniProt Protein Name: Potassium voltage-gated channel subfamily A member 6
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNA6
• UniProt Entry Name: KCNA6_HUMAN

NCBI Description

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class. The coding region of this gene is intronless, and the gene is clustered with genes KCNA1 and KCNA5 on chromosome 12. [provided by RefSeq, Jul 2008]

Uniprot Description

Kv1.6: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.6/KCNA6 sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: voltage-gated potassium channel activity; delayed rectifier potassium channel activity

Biological Process: synaptic transmission; protein homooligomerization; potassium ion transport