Rabbit Kip2, p57 Polyclonal Antibody

Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)

Cat. Num. AAA618568

• Reactivity: Bovine, Human, Mouse
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Kip2; p57; Polyclonal Antibody; p57 (cdk Inhibitor; Cyclin Dependent Kinase Inhibitor); Anti -Kip2; anti-Kip2; p57 antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes p57Kip2. Species Crossreactivity: Human, Bovine and Mouse.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
• Sequence: Near C-terminus

• Applicable Applications for anti-Kip2, p57 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Immunohistology: (Formalin/paraffin) (Use Ab at 10-20ug/ml for 30 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
• Immunogen: A synthetic peptide from human p57Kip2 protein.
• Cellular Localization: Nuclear
• Positive Control: Colon carcinomas or placenta
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Kip2, p57 antibody

p57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms' tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms.

Product Categories/Family for anti-Kip2, p57 antibody

Antibodies; Abs to Cancer Markers