Rabbit anti-Human KIF14 Polyclonal Antibody | anti-KIF14 antibody

KIF14 Antibody

Cat. Num. AAA7050091

• Gene Names: KIF14; MKS12
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: KIF14; Polyclonal Antibody; KIF14 Antibody; Kinesin-like protein KIF14Curated; anti-KIF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1157

• Applicable Applications for anti-KIF14 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Kinesin-like protein KIF14 protein (261-531AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-48559 / sc-48560 / sc-48561 / sc-48562
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using MBS7050091 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7050091 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HepG2 cells using MBS7050091 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-KIF14 antibody

Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity (By similarity). Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis (PubMed: 24784001, PubMed: 16648480). During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively (PubMed: 16431929). Regulates cell growth through regulation of cell cycle progression and cytokinesis (PubMed: 24854087). During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1 (PubMed: 24854087). During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division (By similarity). Also is required for chromosome congression and alignment during mitotic cell cycle process (PubMed: 15843429). Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules (PubMed: 23209302).

NCBI and Uniprot Product Information

• NCBI GI #: 795881084
• NCBI GeneID: 9928
• NCBI Accession #: NP_001292721.1
• NCBI GenBank Nucleotide #: NM_001305792.1
• UniProt Accession #: Q15058; Q14CI8; Q4G0A5; Q5T1W3
• Molecular Weight: 186,492 Da
• NCBI Official Full Name: kinesin-like protein KIF14 isoform 2
• NCBI Official Synonym Full Names: kinesin family member 14
• NCBI Official Symbol: KIF14
• NCBI Official Synonym Symbols: MKS12
• NCBI Protein Information: kinesin-like protein KIF14
• UniProt Protein Name: Kinesin-like protein KIF14
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF14
• UniProt Entry Name: KIF14_HUMAN

NCBI Description

This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

KIF14: Plays an essential role in cytokinesis. Belongs to the kinesin-like protein family.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytosol; kinesin complex; membrane; microtubule; midbody; plasma membrane; spindle midzone

Molecular Function: ATP binding; ATPase activity; microtubule binding; microtubule motor activity; PDZ domain binding; plus-end-directed microtubule motor activity; protein binding; protein kinase binding; tubulin binding

Biological Process: activation of protein kinase activity; cell proliferation in forebrain; cerebellar cortex development; cerebellar granular layer structural organization; cerebellar Purkinje cell layer structural organization; cerebral cortex development; cytoskeleton-dependent intracellular transport; establishment of protein localization; hippocampus development; microtubule depolymerization; microtubule-based movement; mitotic metaphase plate congression; negative regulation of apoptosis; negative regulation of integrin activation; negative regulation of neuron apoptosis; olfactory bulb development; positive regulation of cell proliferation; positive regulation of cytokinesis; proteasomal ubiquitin-dependent protein catabolic process; regulation of cell adhesion; regulation of cell growth; regulation of cell migration; regulation of G2/M transition of mitotic cell cycle; regulation of myelination; regulation of neuron apoptosis; regulation of Rap protein signal transduction; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Disease: Meckel Syndrome 12