Rabbit anti-Human KIF14 Polyclonal Antibody | anti-KIF14 antibody

KIF14 Rabbit pAb

Cat. Num. AAA4760822

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: KIF14; Polyclonal Antibody; KIF14 Rabbit pAb; kinesin family member 14; MKS12; anti-KIF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.7mg/ml (varies by lot)

• Applicable Applications for anti-KIF14 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; IHC-P: 1:25-1:100
• Immunogen: Synthetic peptide of human KIF14
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of KIF14 in K562 cell lysate using KIF14 Rabbit pAb at dilution 1/400)

Immunohistochemistry (IHC)

(Immunohistochemistry of KIF14 in paraffin-embedded Human tonsil tissue using KIF14 Rabbit pAb at dilution 1/35. Predicted cell location: Cytoplasm and Cell membrane)

Related Product Information for anti-KIF14 antibody

This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 119611721
• NCBI GeneID: 9928
• UniProt Accession #: Q15058; Q14CI8; Q4G0A5; Q5T1W3
• Molecular Weight: 186,492 Da
• NCBI Official Full Name: kinesin family member 14, isoform CRA_a
• NCBI Official Synonym Full Names: kinesin family member 14
• NCBI Official Symbol: KIF14
• NCBI Protein Information: kinesin-like protein KIF14
• UniProt Protein Name: Kinesin-like protein KIF14
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF14
• UniProt Synonym Gene Names: KIAA0042
• UniProt Entry Name: KIF14_HUMAN

NCBI Description

KIF14 is a member of the kinesin superfamily of microtubule-associated motors (see MIM 148760) that play important roles in intracellular transport and cell division (Nakagawa et al., 1997 [PubMed 9275178]).[supplied by OMIM, Mar 2008]

Uniprot Description

KIF14: Plays an essential role in cytokinesis. Belongs to the kinesin-like protein family.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: microtubule; kinesin complex; membrane; cytoplasm; plasma membrane; spindle midzone; midbody; nucleus

Molecular Function: tubulin binding; protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; protein kinase binding; ATP binding; PDZ domain binding

Biological Process: positive regulation of cytokinesis; regulation of Rap protein signal transduction; regulation of cell adhesion; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; cerebellar granular layer structural organization; regulation of myelination; cell proliferation in forebrain; olfactory bulb development; hippocampus development; cytoskeleton-dependent intracellular transport; negative regulation of integrin activation; regulation of cell migration; mitotic metaphase plate congression; microtubule-based movement; cerebellar cortex development; establishment of protein localization; regulation of G2/M transition of mitotic cell cycle; regulation of neuron apoptosis; positive regulation of cell proliferation; activation of protein kinase activity; microtubule depolymerization; negative regulation of neuron apoptosis; cerebral cortex development; regulation of cell growth; cerebellar Purkinje cell layer structural organization; negative regulation of apoptosis

Disease: Meckel Syndrome 12