Western Blot (WB)
(Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.)
Rabbit anti-Human, Mouse T-bet Polyclonal Antibody | anti-T-bet antibody
T-bet Antibody
Gene Names
Tbx21; TBT1; Tbet; Tblym
Reactivity
Human, Mouse
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified.
Synonyms
T-bet; Polyclonal Antibody; T-bet Antibody; T bet antibody; T box 21 antibody; T box expressed in T cells antibody; T box protein 21 antibody; T box transcription factor TBX21 antibody; T cell specific T box transcription factor antibody; T cell specific T box transcription factor T bet antibody; T PET antibody; T-box protein 21 antibody; T-box transcription factor TBX21 antibody; T-cell-specific T-box transcription factor T-bet antibody; TBET antibody; TBLYM antibody; TBX 21 antibody; Tbx21 antibody; TBX21_HUMAN antibody; TPET antibody; Transcription factor TBLYM antibody; anti-T-bet antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified.
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-T-bet antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
FC/FACS: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
Synthetic peptide within human T-bet aa 480-530.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Jurkat cells with T-bet antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Jurkat cells with T-bet antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-T-bet antibody
T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-gamma. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.
Product Categories/Family for anti-T-bet antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,852 Da
NCBI Official Full Name
T-box transcription factor TBX21
NCBI Official Synonym Full Names
T-box 21
NCBI Official Symbol
Tbx21
NCBI Official Synonym Symbols
TBT1; Tbet; Tblym
NCBI Protein Information
T-box transcription factor TBX21; T-box protein 21; transcription factor TBLYM; T-cell-specific T-box transcription factor T-bet
UniProt Protein Name
T-box transcription factor TBX21
UniProt Gene Name
Tbx21
UniProt Synonym Gene Names
Tbet; Tblym; T-box protein 21
UniProt Entry Name
TBX21_MOUSE
Similar Products
Product Notes
The T-bet tbx21 (Catalog #AAA9435389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The T-bet Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's T-bet can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the T-bet tbx21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T-bet, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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