Rabbit anti-Human LETM2 Polyclonal Antibody | anti-LETM2 antibody

LETM2, NT (LETM2, LETM1 domain-containing protein LETM2, mitochondrial, LETM1 and EF-hand domain-containing protein 2, Leucine zipper-EF-hand-containing transmembrane protein 1-like) (AP)

Cat. Num. AAA6315745

• Gene Names: LETM2; SLC55A2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LETM2; Polyclonal Antibody; NT (LETM2; LETM1 domain-containing protein LETM2; mitochondrial; LETM1 and EF-hand domain-containing protein 2; Leucine zipper-EF-hand-containing transmembrane protein 1-like) (AP); Leucine zipper-EF-hand-containing transmembrane protein 1-like; anti-LETM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 396

• Applicable Applications for anti-LETM2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LETM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the N-terminal region of human LETM2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LETM2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the LETM2 antibody detected the LETM2 protein (arrow).)

Immunohistochemistry (IHC)

(LETM2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LETM2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LETM2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-LETM2 antibody

Antibodies; Transmembrane Proteins

References

Stec, I., et al. Genomics 76 (1-3), 5-8 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 21389523
• NCBI GeneID: 137994
• NCBI Accession #: NP_653253.1
• NCBI GenBank Nucleotide #: NP_653253.1
• UniProt Accession #: Q2VYF4
• NCBI Official Full Name: LETM1 domain-containing protein LETM2, mitochondrial isoform 2
• NCBI Official Synonym Full Names: leucine zipper and EF-hand containing transmembrane protein 2
• NCBI Official Symbol: LETM2
• NCBI Official Synonym Symbols: SLC55A2
• NCBI Protein Information: LETM1 domain-containing protein LETM2, mitochondrial
• UniProt Protein Name: LETM1 domain-containing protein LETM2, mitochondrial
• Protein Family: LETM1 domain-containing protein
• UniProt Gene Name: LETM2
• UniProt Entry Name: LETM2_HUMAN