anti-Human, Mouse CDK8 Antibody | anti-CDK8 antibody

CDK8 Antibody

Cat. Num. AAA854040

• Gene Names: CDK8; K35
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CDK8; CDK8 Antibody; Cell division protein kinase 8; EC 2.7.11.22; EC 2.7.11.23; Protein kinase K35; anti-CDK8 antibody

• Reactivity: Human, Mouse
• Specificity: CDK8 antibody detects endogenous levels of total CDK8 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 464

• Applicable Applications for anti-CDK8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from N-terminal of human CDK8.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using CDK8 antibody.)

Product Categories/Family for anti-CDK8 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Tassan J.-P., Proc. Natl. Acad. Sci. U.S.A. 92:8871-8875(1995). Fryer C.J., Mol. Cell 16:509-520(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4502745
• NCBI GeneID: 1024
• NCBI Accession #: NP_001251.1
• NCBI GenBank Nucleotide #: NM_001260.2
• UniProt Accession #: P49336; Q5VUF3; Q6ISB5
• Molecular Weight: 53,156 Da
• NCBI Official Full Name: cyclin-dependent kinase 8 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase 8
• NCBI Official Symbol: CDK8
• NCBI Official Synonym Symbols: K35
• NCBI Protein Information: cyclin-dependent kinase 8
• UniProt Protein Name: Cyclin-dependent kinase 8
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK8

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.