Rabbit KATNA1 Polyclonal Antibody | anti-KATNA1 antibody

KATNA1 Antibody

Cat. Num. AAA9608507

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: KATNA1; Polyclonal Antibody; KATNA1 Antibody; Katanin p60 ATPase containing subunit A1; Katanin p60 ATPase-containing subunit A1; Katanin p60 subunit A1; KTNA1_HUMAN; OTTHUMP00000017392; OTTHUMP00000042860; p60 katanin; anti-KATNA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KATNA1 antibody detects endogenous levels of total KATNA1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 311

• Applicable Applications for anti-KATNA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human KATNA1
• Subcellular Location: Cytoplasm. Cytoplasm > Cytoskeleton > Centrosome. Cytoplasm > Cytoskeleton > Spindle Pole. Predominantly Cytoplasmic. Also localized to the interphase centrosome. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of KATNA1 using Jurkat whole cell lysates)

Related Product Information for anti-KATNA1 antibody

Function: Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.
Subunit Structure: Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit (PubMed:10751153). Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM (By similarity). Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex) (PubMed:19287380). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42 (PubMed:19261606). Interacts with KATNB1 and KATNBL1 (PubMed:26929214). Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches (PubMed:24486153, PubMed:28386021).
Post-translational Modifications: Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation. Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.
Similarity: The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization. Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 323276651
• NCBI GeneID: 11104
• NCBI Accession #: NP_001191005.1
• NCBI GenBank Nucleotide #: NM_001204076.1
• UniProt Accession #: O75449; Q5TFA8; Q5TFA9; Q86VN2; Q9NU52; E1P5A3
• Molecular Weight: Observed: 56 kDa; Predicted: 56 kDa
• NCBI Official Full Name: katanin p60 ATPase-containing subunit A1 isoform 2
• NCBI Official Synonym Full Names: katanin catalytic subunit A1
• NCBI Official Symbol: KATNA1
• NCBI Protein Information: katanin p60 ATPase-containing subunit A1
• UniProt Protein Name: Katanin p60 ATPase-containing subunit A1
• UniProt Gene Name: KATNA1
• UniProt Synonym Gene Names: Katanin p60 subunit A1

NCBI Description

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. This gene encodes the p80 subunit. This protein is a member of the AAA family of ATPases. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2011]

Uniprot Description

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.