Typical Testing Data/Standard Curve (for reference only)
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Human Annexin A8 (ANXA8) ELISA Kit | ANXA8 elisa kit
Human Annexin A8 (ANXA8) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: ANXA8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANXA8 antibody and an ANXA8-HRP conjugate. The assay sample and buffer are incubated together with ANXA8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA8 concentration since ANXA8 from samples and ANXA8-HRP conjugate compete for the anti-ANXA8 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA8 from the sample, fewer sites are left to bind ANXA8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA8 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the annexin family of evolutionarily conserved Ca2+ and phospholipid binding proteins. The encoded protein may function as an an anticoagulant that indirectly inhibits the thromboplastin-specific complex. Overexpression of this gene has been associated with acute myelocytic leukemia. A highly similar duplicated copy of this gene is found in close proximity on the long arm of chromosome 10. [provided by RefSeq, Jul 2008]
Uniprot Description
ANXA8: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Belongs to the annexin family.
Chromosomal Location of Human Ortholog: 10q11.22
Cellular Component: late endosome membrane; plasma membrane; cytosol
Molecular Function: actin filament binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; calcium-dependent phospholipid binding; calcium ion binding; phosphatidylinositol-3,4-bisphosphate binding
Biological Process: endosome organization and biogenesis; blood coagulation; endosome transport
Research Articles on ANXA8
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Product Notes
The Human ANXA8 anxa8 (Catalog #AAA7239665) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7239665 ELISA Kit recognizes Human ANXA8. It is sometimes possible for the material contained within the vial of "Annexin A8 (ANXA8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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