Typical Testing Data/Standard Curve (for reference only)
Porcine Amiloride-sensitive sodium channel subunit alpha (SCNN1A) ELISA Kit | SCNN1A elisa kit
Porcine Amiloride-sensitive sodium channel subunit alpha (SCNN1A) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SCNN1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SCNN1A antibody and an SCNN1A-HRP conjugate. The assay sample and buffer are incubated together with SCNN1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCNN1A concentration since SCNN1A from samples and SCNN1A-HRP conjugate compete for the anti-SCNN1A antibody binding site. Since the number of sites is limited, as more sites are occupied by SCNN1A from the sample, fewer sites are left to bind SCNN1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCNN1A concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]
Uniprot Description
ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Transporter, ion channel; Channel, sodium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: cortical actin cytoskeleton; integral to plasma membrane; apical plasma membrane; plasma membrane; cytosol; external side of plasma membrane
Molecular Function: amiloride-sensitive sodium channel activity; protein binding; WW domain binding; actin binding
Biological Process: sensory perception of taste; response to stimulus; sodium ion transport; sodium ion homeostasis; multicellular organismal water homeostasis; excretion; transmembrane transport
Disease: Bronchiectasis With Or Without Elevated Sweat Chloride 2; Pseudohypoaldosteronism, Type I, Autosomal Recessive
Research Articles on SCNN1A
Similar Products
Product Notes
The Porcine SCNN1A scnn1a (Catalog #AAA7218398) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218398 ELISA Kit recognizes Porcine SCNN1A. It is sometimes possible for the material contained within the vial of "Amiloride-sensitive sodium channel subunit alpha (SCNN1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
