Western Blot (WB)
(Western Blot (WB) analysis of Raly (R103) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Rabbit Raly Polyclonal Antibody | anti-Raly antibody
Raly (R103) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Raly; Polyclonal Antibody; Raly (R103) Polyclonal Antibody; RNA-binding protein Raly; Autoantigen p542; Heterogeneous nuclear ribonucleoprotein C-like 2; hnRNP core protein C-like 2; hnRNP associated with lethal yellow protein homolog; RALY; HNRPCL2; P542; anti-Raly antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Raly (R103) polyclonal antibody detects endogenous levels of Raly protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
290
Applicable Applications for anti-Raly antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 70-120 of Human Raly.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Raly (R103) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Raly (R103) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Related Product Information for anti-Raly antibody
Raly, also known as hnRNP associated with lethal yellow homolog, autoantigen p542 or heterogeneous nuclear ribonucleoprotein C-like 2 (hnRNPCL2), is a 306 amino acid protein belonging to the RRM HNRPC family. Localized to the nucleus, Raly is thought to be an RNA-binding protein involved in pre-mRNA splicing. Phosphorylated upon DNA damage, Raly has been found to be a subunit of the spliceosome C complex. Raly is also an autoantigen that is crossreactive with an Epstein-Barr virus protein. Raly is expressed at higher levels in pancreas, liver, skeletal muscle, lung, brain, heart and kidney with lower levels found in placenta. Produced by alternative splicing, Raly is expressed as two isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 32kDa
NCBI Official Full Name
RNA-binding protein Raly isoform 2
UniProt Protein Name
RNA-binding protein Raly
Protein Family
UniProt Gene Name
RALY
UniProt Synonym Gene Names
HNRPCL2; P542; hnRNP core protein C-like 2
UniProt Entry Name
RALY_HUMAN
Uniprot Description
RALY: Probable-RNA binding protein. Could be a heterogeneous nuclear ribonucleoprotein (hnRNP). May be involved in pre-mRNA splicing. Belongs to the RRM HNRPC family. RALY subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Spliceosome; RNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 20q11.21-q11.23
Cellular Component: nucleus
Molecular Function: protein binding; RNA binding; nucleotide binding
Biological Process: nuclear mRNA splicing, via spliceosome
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Product Notes
The Raly raly (Catalog #AAA3013866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Raly (R103) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Raly can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the Raly raly for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Raly, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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