Rabbit anti-Human ITPKB Polyclonal Antibody | anti-ITPKB antibody

ITPKB, NT (ITPKB, Inositol-trisphosphate 3-kinase B, Inositol 1,4,5-trisphosphate 3-kinase B) (PE)

Cat. Num. AAA6311432

• Gene Names: ITPKB; IP3K; IP3KB; PIG37; IP3K-B; IP3-3KB
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ITPKB; Polyclonal Antibody; NT (ITPKB; Inositol-trisphosphate 3-kinase B; Inositol 1; 4; 5-trisphosphate 3-kinase B) (PE); 5-trisphosphate 3-kinase B; anti-ITPKB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ITPKB antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ITPKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31~61 amino acids from the N-terminal region of human ITPKB.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ITPKB (arrow) using rabbit polyclonal ITPKB Antibody (R38). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ITPKB gene.)

Immunohistochemistry (IHC)

(ITPKB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ITPKB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ITPKB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ITPKB antibody

ITPKB regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity.

Product Categories/Family for anti-ITPKB antibody

Antibodies; Protein Kinases

References

Dewaste, V., et al., Biochem. Biophys. Res. Commun. 291(2):400-405 (2002). Woodring, P.J., et al., J. Biol. Chem. 272(48):30447-30454 (1997). Erneux, C., et al., Genomics 14(2):546-547 (1992). Takazawa, K., et al., Biochem. J. 272(1):107-112 (1990). Takazawa, K., et al., Biochem. J. 278 (PT 3), 883-886 (1991) (): ().

NCBI and Uniprot Product Information

• NCBI GI #: 194272161
• NCBI GeneID: 3707
• NCBI Accession #: NP_002212
• NCBI GenBank Nucleotide #: NM_002221.3
• UniProt Accession #: P27987; Q5VWL9; Q5VWM0; Q96BZ2; Q96JS1; Q9UH47
• Molecular Weight: 67,387 Da
• NCBI Official Full Name: inositol-trisphosphate 3-kinase B
• NCBI Official Synonym Full Names: inositol-trisphosphate 3-kinase B
• NCBI Official Symbol: ITPKB
• NCBI Official Synonym Symbols: IP3K; IP3KB; PIG37; IP3K-B; IP3-3KB
• NCBI Protein Information: inositol-trisphosphate 3-kinase B
• UniProt Protein Name: Inositol-trisphosphate 3-kinase B
• Protein Family: Inositol-trisphosphate 3-kinase
• UniProt Gene Name: ITPKB
• UniProt Synonym Gene Names: IP3 3-kinase B; IP3K B
• UniProt Entry Name: IP3KB_HUMAN

NCBI Description

The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ITPKB: The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis; EC 2.7.1.127; Carbohydrate Metabolism - inositol phosphate; Kinase, lipid

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: cytosol

Molecular Function: calmodulin binding; protein binding; inositol trisphosphate 3-kinase activity; ATP binding

Biological Process: cell surface receptor linked signal transduction; inositol phosphate metabolic process; MAPKKK cascade; positive regulation of Ras protein signal transduction; signal transduction; positive regulation of alpha-beta T cell differentiation; positive thymic T cell selection