Western Blot (WB)
(Western blot analysis of SKIP expression in mouse brain (A), rat brain (B) whole cell lysates.)
Rabbit anti-Human SKIP Polyclonal Antibody | anti-INPP5K antibody
Anti-SKIP Antibody
Western Blot (WB)
(Western blot analysis of SKIP expression in mouse brain (A), rat brain (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of SKIP expression in mouse brain (A), rat brain (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of SKIP staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of SKIP staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]
Uniprot Description
INPP5K: Inositol 5-phosphatase which acts on inositol 1,4,5- trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5- trisphosphate. May negatively regulate assembly of the actin cytoskeleton. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.56; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; membrane; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; ruffle; trans-Golgi network
Molecular Function: inositol bisphosphate phosphatase activity; inositol trisphosphate phosphatase activity; inositol-polyphosphate 5-phosphatase activity; lipid phosphatase activity; phosphoinositide 5-phosphatase activity; protein binding; vasopressin receptor activity
Biological Process: actin cytoskeleton organization and biogenesis; cellular response to hormone stimulus; cellular response to insulin stimulus; dephosphorylation; G-protein coupled receptor protein signaling pathway; glucose homeostasis; in utero embryonic development; inositol phosphate dephosphorylation; negative regulation of calcium ion transport; negative regulation of dephosphorylation; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; negative regulation of protein kinase B signaling cascade; negative regulation of retroviral genome replication; negative regulation of stress fiber formation; negative regulation of transcription, DNA-dependent; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; positive regulation of transcription, DNA-dependent; regulation of glycogen biosynthetic process
Research Articles on INPP5K
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Product Notes
The INPP5K inpp5k (Catalog #AAA8245231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SKIP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SKIP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the INPP5K inpp5k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SKIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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