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Western Blot (WB) (Western blot analysis of extracts from HuvEc cells, using CHST10 antibody.)

Rabbit anti-Human CHST10 Polyclonal Antibody | anti-CHST10 antibody

CHST10 Antibody

Gene Names
CHST10; HNK1ST; HNK-1ST
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CHST10; Polyclonal Antibody; CHST10 Antibody; Carbohydrate sulfotransferase 10; EC 2.8.2.-; HNK-1 sulfotransferase; HNK1ST; HNK-1ST; anti-CHST10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total CHST10 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
356
Applicable Applications for anti-CHST10 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human CHST10.
Target Name
CHST10
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HuvEc cells, using CHST10 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HuvEc cells, using CHST10 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CHST10 antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CHST10 antibody.)
Related Product Information for anti-CHST10 antibody
Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.Ong E., J. Biol. Chem. 273:5190-5195(1998).Yu W., Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.Ong E., J. Biol. Chem. 274:25608-25612(1999).
Product Categories/Family for anti-CHST10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,207 Da
NCBI Official Full Name
carbohydrate sulfotransferase 10
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 10
NCBI Official Symbol
CHST10
NCBI Official Synonym Symbols
HNK1ST; HNK-1ST
NCBI Protein Information
carbohydrate sulfotransferase 10
UniProt Protein Name
Carbohydrate sulfotransferase 10
UniProt Gene Name
CHST10
UniProt Synonym Gene Names
HNK-1ST; HNK1ST; HuHNK-1ST
UniProt Entry Name
CHSTA_HUMAN

NCBI Description

This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.[provided by RefSeq, Feb 2011]

Uniprot Description

HNK1ST: Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis. Belongs to the sulfotransferase 2 family.

Protein type: EC 2.8.2.-; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane

Molecular Function: sulfotransferase activity; HNK-1 sulfotransferase activity

Biological Process: long-term memory; learning; cell adhesion; carbohydrate biosynthetic process

Research Articles on CHST10

Similar Products

Product Notes

The CHST10 chst10 (Catalog #AAA9408233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHST10 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHST10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100. Researchers should empirically determine the suitability of the CHST10 chst10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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