Rabbit Itk Polyclonal Antibody | anti-Itk antibody

Mouse Itk Antibody (N-term)

Cat. Num. AAA9207715

• Gene Names: Itk; Emt; Tsk; Tcsk
• Reactivity: Mouse (Predicted Reactivity: Human)
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Itk; Polyclonal Antibody; Mouse Itk Antibody (N-term); Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; T-cell-specific kinase; Emt; Tlk; Tsk; anti-Itk antibody

• Host: Rabbit
• Reactivity: Mouse (Predicted Reactivity: Human)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Itk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 156-190 amino acids from the N-terminal region of human Mouse Itk.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 625

• Applicable Applications for anti-Itk antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from mouse thymus and mouse spleen tissue lysate (from left to right), using Mouse Itk Antibody (N-term). MBS9207715 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-Itk antibody

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

Product Categories/Family for anti-Itk antibody

Crown Antibodies; Signal Transduction

References

Siliciano J.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:11194-11198(1992).
Heyeck S.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:669-673(1993).
Yamada N.,et al.Biochem. Biophys. Res. Commun. 192:231-240(1993).
Ogata M.,et al.Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases.
Liao X.C.,et al.Immunity 3:757-769(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 530354648
• NCBI GeneID: 16428
• NCBI Accession #: NP_001268894.1
• NCBI GenBank Nucleotide #: NM_001281965.1
• UniProt Accession #: Q03526
• Molecular Weight: 72292
• NCBI Official Full Name: tyrosine-protein kinase ITK/TSK isoform 1
• NCBI Official Synonym Full Names: IL2 inducible T cell kinase
• NCBI Official Symbol: Itk
• NCBI Official Synonym Symbols: Emt; Tsk; Tcsk
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Itk
• UniProt Synonym Gene Names: Emt; Tlk; Tsk
• UniProt Entry Name: ITK_MOUSE

Uniprot Description

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. The protein contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; Tec family

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; intercellular junction

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: adaptive immune response; peptidyl-tyrosine phosphorylation; immune system process; cytokine production; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; interleukin-4 production; phospholipase C activation; interferon-gamma production; innate immune response; NK T cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway