Rabbit anti-Human FBLIM1 Polyclonal Antibody | anti-FBLIM1 antibody

FBLIM1, CT (FBLIM1, FBLP1, Filamin-binding LIM protein 1, Migfilin, Mitogen-inducible 2-interacting protein) (FITC)

Cat. Num. AAA6298134

• Gene Names: FBLIM1; CAL; FBLP1; FBLP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBLIM1; Polyclonal Antibody; CT (FBLIM1; FBLP1; Filamin-binding LIM protein 1; Migfilin; Mitogen-inducible 2-interacting protein) (FITC); Mitogen-inducible 2-interacting protein; anti-FBLIM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FBLIM1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FBLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-331 amino acids from the C-terminal region of human FBLIM1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBLIM1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBLIM1 antibody detected the FBLIM1 protein (arrow).)

Immunohistochemistry (IHC)

(FBLIM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBLIM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-FBLIM1 antibody

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-FBLIM1 antibody

Antibodies; Binding Proteins

References

Zhao, J., et al. J. Biol. Chem. 284(49):34308-34320(2009). Ithychanda, S.S., et al. J. Biol. Chem. 284(7):4713-4722(2009). Lad, Y., et al. J. Biol. Chem. 283(50):35154-35163(2008). Lai-Cheong, J.E., et al. J. Invest. Dermatol. 128(9):2156-2165(2008). Papachristou, D.J., et al. Histopathology 51(4):499-508(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 66932986
• NCBI GeneID: 54751
• NCBI Accession #: NP_001019386.1; NP_060026.2
• NCBI GenBank Nucleotide #: NM_001024215.1
• UniProt Accession #: Q8WUP2; Q5VVE0; Q5VVE1; Q8IX23; Q96T00; Q9UFD6; B3KNY0
• Molecular Weight: 30,770 Da
• NCBI Official Full Name: filamin-binding LIM protein 1 isoform b
• NCBI Official Synonym Full Names: filamin binding LIM protein 1
• NCBI Official Symbol: FBLIM1
• NCBI Official Synonym Symbols: CAL; FBLP1; FBLP-1
• NCBI Protein Information: filamin-binding LIM protein 1; CSX-associated LIM; MIG2-interacting protein; filamin-binding LIM protein-1; migfilin; mitogen-inducible 2 interacting protein; mitogen-inducible 2-interacting protein
• UniProt Protein Name: Filamin-binding LIM protein 1
• Protein Family: Filamin-binding LIM protein
• UniProt Gene Name: FBLIM1
• UniProt Synonym Gene Names: FBLP1; FBLP-1
• UniProt Entry Name: FBLI1_HUMAN

NCBI Description

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: focal adhesion; stress fiber; cell cortex; cytosol

Molecular Function: zinc ion binding; filamin binding

Biological Process: regulation of cell shape; cell-cell adhesion; regulation of integrin activation