Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue slide using MBS7612020(ITK antibody) at dilution of 1:100)
Rabbit anti-Human ITK Polyclonal Antibody | anti-ITK antibody
anti-ITK antibody
Gene Names
ITK; EMT; LYK; LPFS1; PSCTK2
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
>=95% as determined by SDS-PAGE. Immunogen affinity purified
Synonyms
ITK; Polyclonal Antibody; anti-ITK antibody; EMT; IL2 inducible T cell kinase; Kinase EMT; LYK; PSCTK2; T cell specific kinase; Tyrosine protein kinase Lyk
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>=95% as determined by SDS-PAGE. Immunogen affinity purified
Form/Format
Liquid. PBS with 0.02% sodium azide and 50% glycerol pH7.3
Applicable Applications for anti-ITK antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB: 1:200-1:2000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
IL2-inducible T-cell kinase
Preparation and Storage
Store at -20 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue slide using MBS7612020(ITK antibody) at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney tissue slide using MBS7612020(ITK antibody) at dilution of 1:100)
Western Blot (WB)
(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7612020(ITK antibody) at dilution of 1:1000)
Western Blot (WB)
(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7612020(ITK antibody) at dilution of 1:1000)
Related Product Information for anti-ITK antibody
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells(APC) activate T-cell receptor(TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells(NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
620
NCBI Official Full Name
Tyrosine-protein kinase ITK/TSK
NCBI Official Synonym Full Names
IL2-inducible T-cell kinase
NCBI Official Symbol
ITK
NCBI Official Synonym Symbols
EMT; LYK; LPFS1; PSCTK2
NCBI Protein Information
tyrosine-protein kinase ITK/TSK; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; homolog of mouse T-cell itk/tsk; interleukin-2-inducible T cell kinase; interleukin-2-inducible T
UniProt Protein Name
Tyrosine-protein kinase ITK/TSK
Protein Family
UniProt Gene Name
ITK
UniProt Synonym Gene Names
EMT; LYK; IL-2-inducible T-cell kinase
UniProt Entry Name
ITK_HUMAN
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Product Notes
The ITK itk (Catalog #AAA7612020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The anti-ITK antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ITK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB: 1:200-1:2000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the ITK itk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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