Rabbit ITK Polyclonal Antibody | anti-ITK antibody

ITK Rabbit Polyclonal

Cat. Num. AAA7600647

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purity: >=95% as determined by SDS-PAGE; Purification: Immunogen affinity purified
• Synonyms: ITK; Polyclonal Antibody; ITK Rabbit Polyclonal; EMT; IL2 inducible T cell kinase; Kinase EMT; LYK; PSCTK2; T cell specific kinase; Tyrosine protein kinase Lyk; anti-ITK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purity: >=95% as determined by SDS-PAGE; Purification: Immunogen affinity purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3

• Applicable Applications for anti-ITK antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: IL2-inducible T-cell kinase
• Preparation and Storage: Store at -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7600647 (ITK antibody) at dilution of 1:1000)

Related Product Information for anti-ITK antibody

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells(APC) activate T-cell receptor(TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells(NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 399658
• NCBI GeneID: 3702
• Molecular Weight: Observed MW: 68-72kd
• NCBI Official Full Name: ITK
• NCBI Official Synonym Full Names: IL2 inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. The protein contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: cytosol; extrinsic to internal side of plasma membrane; intercellular junction

Molecular Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; receptor binding

Biological Process: adaptive immune response; cellular defense response; cytokine production; innate immune response; interferon-gamma production; interleukin-4 production; NK T cell differentiation; phospholipase C activation; regulation of cell proliferation; signal transduction; T cell activation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lymphoproliferative Syndrome 1