Rabbit anti-Human, Mouse ITK Polyclonal Antibody | anti-ITK antibody

ITK (Phospho-Tyr512) Antibody

Cat. Num. AAA9502850

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: ITK; Polyclonal Antibody; ITK (Phospho-Tyr512) Antibody; EMT; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; ITK/TSK; Kinase EMT; kinase Itk; LYK; Lyk; T-cell-specific kinase; TLK; TSK; anti-ITK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 620

• Applicable Applications for anti-ITK antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:Y512; M:Y517
• Immunogen: The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512.
• Immunogen Range: 478-527
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

NCBI and Uniprot Product Information

• NCBI GI #: 15718680
• NCBI GeneID: 3702
• NCBI Accession #: NP_005537.3
• NCBI GenBank Nucleotide #: NM_005546.3
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 71 kDa
• NCBI Official Full Name: tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2 inducible T cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 ().