ITK recombinant protein

ITK Protein, Human, Recombinant (Actived By LCK, GST Tag)

Cat. Num. AAA8123628

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Purity: >75% as determined by SDS-PAGE
• Synonyms: ITK; ITK Protein; Human; Recombinant (Actived By LCK; GST Tag); Human ITK Kinase Protein (Actived By LCK; EMT Protein; LPFS1 Protein; LYK Protein; PSCTK2 Protein; IL2-inducible T-cell kinase; ITK recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >75% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH7.5, 25% glycerol, 0.5mM PMSF, 0.5mM TCEP, 0.1mM EDTA, 0.5mM GSH. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer in
• Sequence: Arg352-Leu620

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human ITK (NP_005537.3)(Arg352-Leu620) was expressed with a GST tag at the N-terminus. The purified ITK was actived by LCK.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ITK recombinant protein

Background: IL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family, and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain, and 1 SH3 domain. As an intracellular kinase expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. It regulates the development, function, and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in the regulation of the adaptive immune response. Effects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk of lymphoma.

References

Lee SH, et al. (2011) The association of a single-nucleotide polymorphism of the IL-2 inducible T-cell Kinase gene with asthma. Ann Hum Genet. 75(3):359-69.Yao HL, et al. (2010) Effect of Itk down regulation on cytokines production in Jurkat cell. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 24(5):358-61.Pechloff K, et al. (2010) The fusion kinase ITK-SYK mimics a T cell receptor signal and drives oncogenesis in conditional mouse models of peripheral T cell lymphoma. J Exp Med. 207(5):1031-44.

NCBI and Uniprot Product Information

• NCBI GI #: 15718680
• NCBI GeneID: 3702
• NCBI Accession #: NP_005537.3
• NCBI GenBank Nucleotide #: NM_005546.3
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 71,831 Da
• NCBI Official Full Name: tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2 inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]