Rabbit anti-Human Notch3 Polyclonal Antibody | anti-Notch3 antibody

Notch3, CT (Notch Homolog 3 (Drosophila), hN3, Neurogenic Locus Notch Homolog Protein 3, CADASIL, CASIL) (PE)

Cat. Num. AAA6490340

• Gene Names: NOTCH3; IMF2; CASIL; CADASIL
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Notch3; Polyclonal Antibody; CT (Notch Homolog 3 (Drosophila); hN3; Neurogenic Locus Notch Homolog Protein 3; CADASIL; CASIL) (PE); anti-Notch3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NOTCH3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Notch3 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human NOTCH3 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NOTCH3-Q2306 western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NOTCH3 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-Notch3 antibody

Antibodies; Notch Proteins

References

1. Suwanwela, N., et al., J Med Assoc Thai 86(2):178-182 (2003). 2. Ahearn, E.P., et al., Am. J. Med. Genet. 114(6):652-658 (2002). 3. Bellavia, D., et al., Proc. Natl. Acad. Sci. USA 99(6):3788-3793 (2002). 4. Ito, D., et al., J. Neurol. Neurosurg. Psychiatr. 72(3):382-384 (2002). 5. Joutel, A., et al., Lancet 350(9090):1511-1515 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 134244285
• NCBI GeneID: 4854
• NCBI Accession #: NP_000426
• NCBI GenBank Nucleotide #: NM_000435
• UniProt Accession #: Q9UM47; Q9UEB3; Q9UPL3; Q9Y6L8
• Molecular Weight: 243,631 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 3
• NCBI Official Synonym Full Names: notch 3
• NCBI Official Symbol: NOTCH3
• NCBI Official Synonym Symbols: IMF2; CASIL; CADASIL
• NCBI Protein Information: neurogenic locus notch homolog protein 3; Notch homolog 3
• UniProt Protein Name: Neurogenic locus notch homolog protein 3
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH3
• UniProt Synonym Gene Names: Notch 3
• UniProt Entry Name: NOTC3_HUMAN

Uniprot Description

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cytoplasm; integral to membrane; plasma membrane; extracellular region; cytosol; receptor complex; actin cytoskeleton

Molecular Function: protein binding; enzyme binding; calcium ion binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; forebrain development; neuron fate commitment; Notch receptor processing; gene expression

Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2