Rabbit anti-Human, Mouse ITK Polyclonal Antibody | anti-ITK antibody

ITK (Phospho-Tyr512) Antibody

Cat. Num. AAA9416533

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: ITK; Polyclonal Antibody; ITK (Phospho-Tyr512) Antibody; Emt/Itk/Tsk; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; Kinase EMT; LYK; T-cell-specific kinase; PSCTK2; T-Cell Tyrosine Kinase EMT; EMT; IL-2-inducible T cell kinase; Tyrosine-protein kinase Lyk; anti-ITK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 620

• Applicable Applications for anti-ITK antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human ITK (Phospho-Tyr512)
• Immunogen Type: Peptide
• Modification: Phospho-Tyr512
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis ITK (Phospho-Tyr512) using UV treated 293 whole cell lysates)

Product Categories/Family for anti-ITK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15718680
• NCBI GeneID: 3702
• NCBI Accession #: NP_005537.3
• NCBI GenBank Nucleotide #: NM_005546.3
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 71,831 Da
• NCBI Official Full Name: tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2 inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. Plays an essential role in regulation of the adaptive immune response. Contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Is recruited to the cell membrane following activation of the T-cell receptor (TCR). It is phosphorylated by LCK, followed by autophosphorylation leading to its full activation. Phosphorylates PLCG1. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: cytosol; extrinsic to internal side of plasma membrane

Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; receptor binding

Biological Process: adaptive immune response; cellular defense response; cytokine production; innate immune response; NK T cell differentiation; phospholipase C activation; regulation of cell proliferation; signal transduction; T cell activation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lymphoproliferative Syndrome 1