Western Blot (WB)
(Sample Data: Western blot analysis of IRS1 in PC-3 cell lysate with IRS1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.)
Rabbit anti-Human, Mouse IRS1 Polyclonal Antibody | anti-IRS1 antibody
Anti-IRS1
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
IRS1; Polyclonal Antibody; Anti-IRS1; anti-IRS1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the IRS1 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-IRS1 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
IRS1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human IRS1
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of IRS1 in PC-3 cell lysate with IRS1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of IRS1 in PC-3 cell lysate with IRS1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IRS1 in P815 cells with IRS1 antibody at 2 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IRS1 in P815 cells with IRS1 antibody at 2 ug/ml.)
Related Product Information for anti-IRS1 antibody
IRS1 is the substrate for the insulin tyrosine kinase receptor and is found in a variety of insulin-responsive cells and tissues. IRS1 protein has no intrinsic enzymatic activity but acts as a docking protein, via the SH2 domains, for mediating the insulin downstream signaling events. IRS1 has been shown to associate with the 14-3-3 family of proteins and this could play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (1). IRS1 may be associated with colorectal cancer and diet and related factors may affect the risk by modifying plasma insulin levels. Thus, the inter-individual variation in insulin signaling mediated by IRS1 may play a plausible role in the development of colorectal cancer (2).
Product Categories/Family for anti-IRS1 antibody
References
1. Ogihara, T. et al: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. J. Biol. Chem. 272: 25267-25274, 1997.
2. Slattery, M.L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in Southwestern United States. Breast Cancer Res Treat. 2007.
2. Slattery, M.L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in Southwestern United States. Breast Cancer Res Treat. 2007.
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Product Notes
The IRS1 (Catalog #AAA516027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IRS1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the IRS1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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