Western Blot (WB)
(Sample Data: Western blot analysis of ASK1 in 3T3 cell lysate with ASK1 antibody at (A) 1 and (B) 2 ug/ml.)
Rabbit anti-Human, Mouse ASK1 Polyclonal Antibody | anti-ASK1 antibody
Anti-ASK1
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity chromatography
Synonyms
ASK1; Polyclonal Antibody; Anti-ASK1; anti-ASK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ASK1 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-ASK1 antibody
Western Blot (WB), ELISA
Immunogen
ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ASK1
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of ASK1 in 3T3 cell lysate with ASK1 antibody at (A) 1 and (B) 2 ug/ml.)
Western Blot (WB)
(Sample Data: Western blot analysis of ASK1 in 3T3 cell lysate with ASK1 antibody at (A) 1 and (B) 2 ug/ml.)
Related Product Information for anti-ASK1 antibody
ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha (1). ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation (2).
Product Categories/Family for anti-ASK1 antibody
References
1. Smith, C. Ichijo, H. et al: Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 275: 90-94, 1997.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
2. Nishitoh, H. et al: ASK1 is essential for JNK/SAPK activation by TRAF2. Molec. Cell 2: 389-395, 1998.
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Product Notes
The ASK1 (Catalog #AAA516074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ASK1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ASK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA. Researchers should empirically determine the suitability of the ASK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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