Western Blot (WB)
(Sample Data: Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 ug/ml in either the (A) absence or (B) presence of blocking peptide.)
Rabbit anti-Human, Mouse PTEN Polyclonal Antibody | anti-PTEN antibody
Anti-PTEN
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Affinity chromatography
Synonyms
PTEN; Polyclonal Antibody; Anti-PTEN; anti-PTEN antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the PTEN protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-PTEN antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)
Immunogen
PTEN antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PTEN
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 ug/ml in either the (A) absence or (B) presence of blocking peptide.)
Western Blot (WB)
(Sample Data: Western blot analysis of PTEN in PC-3 cell lysate with PTEN antibody at 1 ug/ml in either the (A) absence or (B) presence of blocking peptide.)
Related Product Information for anti-PTEN antibody
PTEN (phosphatase and tensin homolog) is a tumor suppressor that is frequently mutated in a large number of cancers (1). PTEN has phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity and contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, PTEN preferentially dephosphorylates phosphoinositide substrates and is responsible for negatively regulating the intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells. PTEN functions as a tumor suppressor by negatively regulating the PI3K/AKT signaling pathway (2).
Product Categories/Family for anti-PTEN antibody
References
1. Butler, M.G. et al: Subset of individuals with autism spectrum disorders and extreme macrocephaly associated with germline PTEN tumour suppressor gene mutations. J. Med. Genet. 2005;42(4):318-21.
2. Steck, P.A. et aal: Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet. 1997; 15(4):356-62.
2. Steck, P.A. et aal: Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat. Genet. 1997; 15(4):356-62.
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Product Notes
The PTEN (Catalog #AAA516135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PTEN reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTEN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). Researchers should empirically determine the suitability of the PTEN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTEN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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