Western Blot (WB)
(Sample Data: Western blot analysis of extracts from HEK293 anti-phospho-IRS1 (Ser312) antibody.)
Rabbit IRS1 Polyclonal Antibody | anti-phospho-IRS1 antibody
Anti-phospho-IRS1 (Ser312)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
IRS1; Polyclonal Antibody; Anti-phospho-IRS1 (Ser312); anti-phospho-IRS1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the IRS1 protein phosphorylated at serine 312
Purity/Purification
Affinity chromatography
Form/Format
PBS (pH7.4) 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-phospho-IRS1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser312
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from HEK293 anti-phospho-IRS1 (Ser312) antibody.)
Western Blot (WB)
(Sample Data: Western blot analysis of extracts from HEK293 anti-phospho-IRS1 (Ser312) antibody.)
Related Product Information for anti-phospho-IRS1 antibody
IRS1 is the substrate for the insulin tyrosine kinase receptor and it is expressed in a variety of insulin-responsive tissues. IRS1 is a docking protein with no intrinsic enzymatic activity. Via its SH2 domains, IRS1 facilitates interactions between key players in insulin signaling such as the insulin receptor. 14-3-3 competitively binds with IRS1 against the insulin receptor (1). This competition has implications towards the regulation of insulin sensitivity. IRS1 has been linked to colorectal cancer and diet via the regulation of plasma insulin levels. Thus, variation of IRS1 function may have implications in colorectal cancer (2). IRS1 has several Ser/Thr phosphorylation sites including Ser312, which is sensitive to TNF-alpha or calyculin A treatment (3) and Ser636/639, which is associated with S6K and mTOR activation (4).
Product Categories/Family for anti-phospho-IRS1 antibody
References
1. Ogihara, T. et al: 14-3-3 protein binds to insulin receptor substrate-1, one of the binding sites of which is in the phosphotyrosine binding domain. J. Biol. Chem. 272: 25267-25274, 1997.
2. Slattery, M L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in southwestern United States. Breast Cancer Res Treat. 2007.
3. Gao, Z. et al: Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex. J Biol Chem. 2002 Dec 13;277(50):48115-21.
4. Khamzina, L. et al: Increased activation of the mammalian target of rapamycin pathway in liver and skeletal muscle ofobese rats: possible involvement in obesity-linked insulin resistance. Endocrinology. 2005 Mar;146(3):1473-81.
2. Slattery, M L. et al: Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in southwestern United States. Breast Cancer Res Treat. 2007.
3. Gao, Z. et al: Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex. J Biol Chem. 2002 Dec 13;277(50):48115-21.
4. Khamzina, L. et al: Increased activation of the mammalian target of rapamycin pathway in liver and skeletal muscle ofobese rats: possible involvement in obesity-linked insulin resistance. Endocrinology. 2005 Mar;146(3):1473-81.
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Product Notes
The phospho-IRS1 (Catalog #AAA516030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-IRS1 (Ser312) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IRS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the phospho-IRS1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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