Rabbit IRAK Polyclonal Antibody | anti-IRAK antibody

Anti-IRAK Antibody

Cat. Num. AAA4156756

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purified
• Synonyms: IRAK; Polyclonal Antibody; Anti-IRAK Antibody; anti-IRAK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This IgG fraction antibody is directed against human IRAK protein. The product was purified from monospecific antiserum by DEAE chromatography. A BLAST analysis was used to suggest cross reactivity with IRAK protein from human, mouse and rat based on 100% homology with the immunizing sequence. No cross reactivity occurs against IRAK-2. Reactivity against homologues from other sources is not known.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-IRAK antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: This IgG fraction antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to IRAK protein by western blotting in the appropriate cell lysate or extract.
• Immunogen: This IgG fraction antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human IRAK protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-IRAK antibody

IRAK (Interleukin-1 receptor-associated kinase 1). Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently (1, 2). IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a signaling cascade leading to NF-kB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF) (1, 4).