Mouse anti-Human, Mouse IRAK Monoclonal Antibody | anti-IRAK1 antibody

IRAK Antibody [8F1A7]

Cat. Num. AAA150038

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: IRAK Monoclonal Antibody is immunoaffinity chromotography purified IgG.
• Synonyms: IRAK; Monoclonal Antibody; IRAK Antibody [8F1A7]; IRAK Antibody [8F1A7] : IRAK; pelle; interleukin-1 receptor-associated kinase 1; anti-IRAK1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8F1A7
• Purity/Purification: IRAK Monoclonal Antibody is immunoaffinity chromotography purified IgG.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 712

• Applicable Applications for anti-IRAK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IRAK antibody can be used for Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.
• Buffer: IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IRAK monoclonal antibody can be stored at -20 degree C, stable for one year.

Western Blot (WB)

(Western blot analysis of IRAK in mouse liver lysate with IRAK antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IRAK in rat liver tissue with IRAK antibody at 5 μg/mL.)

Related Product Information for anti-IRAK1 antibody

IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

NCBI and Uniprot Product Information

• NCBI GI #: 8928535
• NCBI GeneID: 3654
• UniProt Accession #: P51617; Q7Z5V4; Q96C06; Q96RL2; D3DWW3; D3DWW4
• Molecular Weight: 68,022 Da
• NCBI Official Full Name: Interleukin-1 receptor-associated kinase 1
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1
• UniProt Entry Name: IRAK1_HUMAN

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; lipid particle; cytosol; nucleus

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; ubiquitin-protein ligase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; stress-activated MAPK cascade; protein ubiquitination; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of MAP kinase activity; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; toll-like receptor 4 signaling pathway; aging; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; innate immune response; regulation of cytokine and chemokine mediated signaling pathway; toll-like receptor 9 signaling pathway; negative regulation of apoptosis