Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1) Recombinant Protein | TGFb1I1 recombinant protein

Recombinant Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1)

Cat. Num. AAA2018805

• Gene Names: TGFB1I1; HIC5; ARA55; HIC-5; TSC-5
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1); Recombinant Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1); TGFb1I1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: Residues: Phe252~Gly461 (Accession # O43294), with two N-terminal Tags, His-tag and T7-tag. The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FVCGGCSTA LGGSSFFEKD GAPFCPECYF ERFSPRCGFC NQPIRHKMVT ALGTHWHPEH FCCVSCGEPF GDEGFHEREG RPYCRRDFLQ LFAPRCQGCQ GPILDNYISA LSALWHPDCF VCRECFAPFS GGSFFEHEGR PLCENHFHAR RGSLCATCGL PVTGRCVSAL GRRFHPDHFT CTFCLRPLTK GSFQERAGKP YCQPCFLKLF G
• Sequence Length: 444

• Applicable Applications for TGFb1I1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 21619705
• NCBI GeneID: 7041
• UniProt Accession #: O43294; Q9BPW3; Q9Y2V5; B2R8D5
• Molecular Weight: 27.4kDa
• NCBI Official Full Name: Transforming growth factor beta 1 induced transcript 1
• NCBI Official Synonym Full Names: transforming growth factor beta 1 induced transcript 1
• NCBI Official Symbol: TGFB1I1
• NCBI Official Synonym Symbols: HIC5; ARA55; HIC-5; TSC-5
• NCBI Protein Information: transforming growth factor beta-1-induced transcript 1 protein; androgen receptor coactivator ARA55; hydrogen peroxide-inducible clone 5 protein; androgen receptor coactivator 55 kDa protein; androgen receptor-associated protein of 55 kDa
• UniProt Protein Name: Transforming growth factor beta-1-induced transcript 1 protein
• Protein Family: Transforming growth factor beta-1-induced transcript 1 protein
• UniProt Gene Name: TGFB1I1
• UniProt Synonym Gene Names: ARA55; Hic-5
• UniProt Entry Name: TGFI1_HUMAN

NCBI Description

This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Hic-5: Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA- binding activity. Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7. Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Up-regulated by TNF and hydrogen peroxide. Expressed in platelets, smooth muscle and prostate stromal cells. Belongs to the paxillin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular matrix; nuclear matrix; focal adhesion; cytoskeleton; cytoplasm; intracellular

Molecular Function: protein binding; Roundabout binding; androgen receptor binding; zinc ion binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; cell fate commitment; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; negative regulation of fat cell differentiation; negative regulation of cell proliferation; morphogenesis of embryonic epithelium; epithelial cell differentiation; response to heat; androgen receptor signaling pathway; cell adhesion; negative regulation of transforming growth factor beta receptor signaling pathway