Rabbit anti-Human, Mouse ECSIT Polyclonal Antibody | anti-ECSIT antibody

Anti-ECSIT Antibody

Cat. Num. AAA4156742

• Gene Names: ECSIT; SITPEC
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity Purified
• Synonyms: ECSIT; Polyclonal Antibody; Anti-ECSIT Antibody; anti-ECSIT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This affinity-purified antibody is directed against human ECSIT protein. The product was purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with ECSIT protein from human and mouse sources based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 431

• Applicable Applications for anti-ECSIT antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 45 kDa in size corresponding to ECSIT protein by western blotting in the appropriate cell lysate or extract.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ECSIT protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-ECSIT antibody

Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 7407498
• NCBI GeneID: 51295
• NCBI Official Full Name: ECSIT
• NCBI Official Synonym Full Names: ECSIT signalling integrator
• NCBI Official Symbol: ECSIT
• NCBI Official Synonym Symbols: SITPEC
• NCBI Protein Information: evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
• Protein Family: Evolutionarily conserved signaling intermediate