Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucyl-cystinyl aminopeptidase (LNPEP) Standard Curve.)
Human Leucyl-Cystinyl Aminopeptidase (LNPEP) ELISA Kit | LNPEP elisa kit
Human Leucyl-Cystinyl Aminopeptidase (LNPEP) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucyl-cystinyl aminopeptidase (LNPEP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucyl-cystinyl aminopeptidase (LNPEP) Standard Curve.)
Background: LNPEP encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. The deduced 944-amino acid polypeptide contains the HEXXH consensus sequence of zinc metallopeptidases as well as an N-terminal hydrophobic region that resembles a transmembrane domain.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
IRAP: a leucyl/cystinyl aminopeptidase. A type II membrane protein and secreted. Resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. Three alternatively spliced isoforms have been described.
Protein type: EC 3.4.11.3; Protease; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q15
Cellular Component: cytoplasmic vesicle membrane; membrane; perinuclear region of cytoplasm; lysosomal membrane; integral to plasma membrane; extracellular region; plasma membrane; intracellular; cytosol
Molecular Function: zinc ion binding; metallopeptidase activity; aminopeptidase activity
Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; cell-cell signaling; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein catabolic process; female pregnancy; proteolysis
Research Articles on LNPEP
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Product Notes
The Human LNPEP lnpep (Catalog #AAA9715466) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715466 ELISA Kit recognizes Human LNPEP. It is sometimes possible for the material contained within the vial of "Leucyl-Cystinyl Aminopeptidase (LNPEP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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