Rabbit anti-Human Insulin-Like Growth Factor I Receptor/Insulin Receptor Polyclonal Antibody | anti-IRS4 antibody

Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1135, Tyr1136/Tyr1162, Tyr1163) (IGF1R, IR)

Cat. Num. AAA611905

• Gene Names: IRS4; IRS-4; PY160
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Insulin-Like Growth Factor I Receptor/Insulin Receptor; Polyclonal Antibody; phosphorylated (Tyr1135; Tyr1136/Tyr1162; Tyr1163) (IGF1R; IR); Anti -Insulin-Like Growth Factor I Receptor/Insulin Receptor; anti-IRS4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human IR/IGF1R when phosphorylated at Tyr1135 and Tyr1136 (IGFR), or Tyr1162 and Tyr1163 (IR). Species sequence homology: mouse and rat (100%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

• Applicable Applications for anti-IRS4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide corresponding to IR/IGF1R that contains tyrosines 1162 and 1163 of the human insulin receptor (IR). The corresponding residues in the IGF1R are 1135 and 1136. Species sequence homology: mouse and rat for both the IR and IGF1R.
• Positive Control: CHO-T cells transfected with a vector containing insulin receptor and stimulated with 100 nM insulin for 10 minutes at 37 degree C.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-IRS4 antibody

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The IR and IGF-1R are heterotetrameric proteins consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain. Insulin/IGF-1 binding to the extracellular domain leads to auto-phosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, leading to tyrosine phosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor.

Product Categories/Family for anti-IRS4 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4504733
• NCBI GeneID: 8471
• NCBI Accession #: NP_003595.1
• NCBI GenBank Nucleotide #: NM_003604.2
• UniProt Accession #: O14654
• Molecular Weight: 133,768 Da
• NCBI Official Full Name: insulin receptor substrate 4
• NCBI Official Synonym Full Names: insulin receptor substrate 4
• NCBI Official Symbol: IRS4
• NCBI Official Synonym Symbols: IRS-4; PY160
• NCBI Protein Information: insulin receptor substrate 4; pp160; phosphoprotein of 160 kDa; 160 kDa phosphotyrosine protein
• UniProt Protein Name: Insulin receptor substrate 4
• Protein Family: Insulin receptor substrate
• UniProt Gene Name: IRS4
• UniProt Synonym Gene Names: IRS-4; py160; pp160
• UniProt Entry Name: IRS4_HUMAN

NCBI Description

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq, Jul 2008]

Uniprot Description

IRS4: Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGFI signaling pathway by suppressing the function of IRS1 and IRS2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity; phosphoinositide 3-kinase binding; insulin receptor binding

Biological Process: regulation of lipid metabolic process; positive regulation of signal transduction; insulin receptor signaling pathway; signal transduction