Rabbit Insulin-Like Growth Factor I Receptor Polyclonal Antibody | anti-IGF1R antibody

Insulin-Like Growth Factor I Receptor, phosphorylated (Tyr1131, Tyr1146) (IGF1R, Insulin Receptor, IR)

Cat. Num. AAA626417

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13; MGC18216; MGC142170; MGC142172
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Insulin-Like Growth Factor I Receptor; Polyclonal Antibody; phosphorylated (Tyr1131; Tyr1146) (IGF1R; Insulin Receptor; IR); Anti -Insulin-Like Growth Factor I Receptor; anti-IGF1R antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of Tyr1131 phosphorylated IGF-I receptor and Tyr1146 phosphorylated insulin receptor. Slightly crossreactivity with activated PDGF, FGF and MET receptors. Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-IGF1R antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50; Immunocytochemistry (ABC): 1:50
• Immunogen: Synthetic phosphopeptide of human IGF1R (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysates from 293 cells treated with 100nM IGF-I for 2 minutes, using Phospho-IGF1R (Tyr1131)/Insulin Receptor (Tyr1146) Antibody (upper) or control IGF1R antibody (lower).)

Western Blot (WB)

(Western Blot analysis of lysates from 3T3-L1 adipocytes treated with 100nM insulin (INS) for indicated times, using Phospho-IGF1R (Tyr1131)/Insulin Receptor (Tyr1146) Antibody (upper) or control IR antibody (lower).)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded human breast carcinoma showing membrane localization, using Phospho-IGF1R (Tyr1131)/Insulin Receptor (Tyr1146) Antibody.)

Immunocytochemistry (ICC)

(Immunocytochemical staining of untreated (left) and IGF-I treated (right) cells, using Phospho-IGF1R (Tyr1131)/Insulin Receptor (Tyr1146) antibody.)

Related Product Information for anti-IGF1R antibody

Type I insulin-like growth factor receptor (IGF1R, IGF-IR) is a transmembrane tyrosine kinase. It is widely expressed among many cell types in fetal and postnatal tissues and many cell lines. Upon binding of its ligands, IGF-I and IGF-II, autophosphorylation of receptors occurs. The triple tyrosine cluster (Tyr1131, Tyr1135 and Tyr1136) within the kinase domain is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for activation of kinases. Insulin receptors (IRs) share significant similarity with IGF-I receptors in structure and function. There is also an equivalent triple tyrosine cluster (Tyr1146, Tyr1150 and Tyr1151) in its activation loop of the kinase domain. Tyrosine autophosphorylation of the receptors is one of the earliest cellular responses to insulin stimulation. The autophosphorylation begins by phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. The full activation of receptor kinase activity depends on the triple tyrosine phosphorylation.

Product Categories/Family for anti-IGF1R antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.3
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2; O43467; Q3B833
• Molecular Weight: 154,793 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13; MGC18216; MGC142170; MGC142172
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-I receptor; OTTHUMP00000194784; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Entry Name: IGF1R_HUMAN

NCBI Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]

Uniprot Description

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; insulin-like growth factor binding; identical protein binding; protein binding; insulin-like growth factor I binding; protein-tyrosine kinase activity; insulin receptor substrate binding; phosphoinositide 3-kinase binding; G-protein alpha-subunit binding; insulin receptor binding; ATP binding; insulin-like growth factor receptor activity

Biological Process: epidermis development; phosphoinositide 3-kinase cascade; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; mammary gland development; positive regulation of MAPKKK cascade; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; axonogenesis; insulin-like growth factor receptor signaling pathway; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; positive regulation of cell migration; negative regulation of apoptosis

Disease: Insulin-like Growth Factor I, Resistance To