Rabbit Insulin-Like Growth Factor I Receptor, beta Polyclonal Antibody | anti-IGF1R antibody

Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)

Cat. Num. AAA630904

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Insulin-Like Growth Factor I Receptor; beta; Polyclonal Antibody; beta (IGF1Rb); Anti -Insulin-Like Growth Factor I Receptor; anti-IGF1R antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human Insulin-Like Growth Factor I Receptor beta at ~95kD. Does not crossreact with insulin receptor. Species Crossreactivity: mouse, rat and monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-IGF1R antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin): 1:2400. Use citrate as unmasking buffer.; Immunoprecipitation: 1:100
• Immunogen: Synthetic peptide corresponding to the carboxy-terminal residues of human IGF1Rb.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of various cell extracts using MBS630904.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast tumor section using MBS630904, showing membrane and cytoplasmic staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using MBS630904.)

Related Product Information for anti-IGF1R antibody

Type I insulin-like growth factor receptor (IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines (1-3). Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant similarity with IGF-I receptors in both structure and function. There is also an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151). Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation (8).

Product Categories/Family for anti-IGF1R antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.3
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2
• Molecular Weight: 154,793 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Synonym Gene Names: IGF-I receptor
• UniProt Entry Name: IGF1R_HUMAN

NCBI Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; identical protein binding; insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; insulin receptor substrate binding; protein-tyrosine kinase activity; G-protein alpha-subunit binding; phosphoinositide 3-kinase binding; ATP binding; insulin receptor binding; insulin-like growth factor receptor activity

Biological Process: phosphoinositide 3-kinase cascade; epidermis development; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; mammary gland development; positive regulation of MAPKKK cascade; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; insulin-like growth factor receptor signaling pathway; axonogenesis; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; positive regulation of cell migration; negative regulation of apoptosis

Disease: Insulin-like Growth Factor I, Resistance To