Rabbit Importin 7 Polyclonal Antibody | anti-IPO7 antibody

Importin 7 antibody

Cat. Num. AAA837645

• Gene Names: IPO7; Imp7; RANBP7
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Importin 7 antibody was purified by antigen-affinity chromatography
• Synonyms: Importin 7; Polyclonal Antibody; Importin 7 antibody; Polyclonal Importin 7; Anti-Importin 7; importin-7; IPO7; RANBP7; importin 7; RAN-binding protein 7; FLJ14581; MGC138673; Importin -7; Imp7; anti-IPO7 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Importin 7 antibody was purified by antigen-affinity chromatography
• Form/Format: Supplied as a concentrated soloution containing 1X PBS, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1038

• Applicable Applications for anti-IPO7 antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF:1:100-1:500; ICC:1:100-1:500; IHC-P: 1:100-1:500; WB: 1:500-1:3000
• Biological Significance: The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions.
• Cross-Reactivity: Human
• Immunogen: Importin 7 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 976 and 1038 of Importin 7
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Importin 7 antibody at 1:200 dilution.)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: JurKat) using a 7.5 % SDS PAGE gel and Importin 7 antibody at a dilution of 1:1000)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded Huh7 xenograft using Importin 7 antibody at a dilution of 1:100)

Related Product Information for anti-IPO7 antibody

Affinity purified Rabbit polyclonal Importin 7 antibody

Product Categories/Family for anti-IPO7 antibody

Signal Transduction; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 92097602
• NCBI GeneID: 10527
• UniProt Accession #: O95373; Q1RMF7; Q9H177; Q9NTE3; A6NNM5; B2R786
• Molecular Weight: 120 kDa
• NCBI Official Full Name: Importin 7
• NCBI Official Synonym Full Names: importin 7
• NCBI Official Symbol: IPO7
• NCBI Official Synonym Symbols: Imp7; RANBP7
• NCBI Protein Information: importin-7
• UniProt Protein Name: Importin-7
• Protein Family: Importin
• UniProt Gene Name: IPO7
• UniProt Synonym Gene Names: RANBP7; Imp7; RanBP7
• UniProt Entry Name: IPO7_HUMAN

NCBI Description

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

IPO7: a beta importin protein. Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin beta-1 subunit. A receptor for nuclear localization signals (NLS) that promotes translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran.

Protein type: Karyopherin; Nuclear import; Nuclear export

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; membrane; cytoplasm; nuclear pore

Molecular Function: protein binding; histone binding; transporter activity; Ran GTPase binding

Biological Process: viral reproduction; regulation of catalytic activity; protein import into nucleus; innate immune response; signal transduction