Rabbit IPO7 Polyclonal Antibody | anti-IPO7 antibody

IPO7 Polyclonal Antibody

Cat. Num. AAA2519181

• Gene Names: IPO7; Imp7; RANBP7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: IPO7; Polyclonal Antibody; IPO7 Polyclonal Antibody; Imp7; RANBP7; anti-IPO7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 2.2mg/mL (varies by lot)
• Sequence Length: 1038

• Applicable Applications for anti-IPO7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:300
• Immunogen: Synthetic peptide of human IPO7
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Hela cell using IPO7 Polyclonal Antibody at dilution of 1:300)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human gastic cancer using IPO7 Polyclonal Antibody at dilution of 1:40)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using IPO7 Polyclonal Antibody at dilution of 1:40)

Related Product Information for anti-IPO7 antibody

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 5453998
• NCBI GeneID: 10527
• NCBI Accession #: NP_006382.1
• NCBI GenBank Nucleotide #: NM_006391.2
• Molecular Weight: 119,517 Da
• NCBI Official Full Name: importin-7
• NCBI Official Synonym Full Names: importin 7
• NCBI Official Symbol: IPO7
• NCBI Official Synonym Symbols: Imp7; RANBP7
• NCBI Protein Information: importin-7; RAN-binding protein 7
• UniProt Protein Name: Importin-7
• Protein Family: Importin
• UniProt Gene Name: IPO7
• UniProt Synonym Gene Names: RANBP7; Imp7; RanBP7
• UniProt Entry Name: IPO7_HUMAN

NCBI Description

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

IPO7: a beta importin protein. Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin beta-1 subunit. A receptor for nuclear localization signals (NLS) that promotes translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran.

Protein type: Nuclear import; Karyopherin; Nuclear export

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; membrane; cytoplasm; nuclear pore

Molecular Function: protein binding; histone binding; transporter activity; Ran GTPase binding

Biological Process: viral reproduction; protein import into nucleus; regulation of catalytic activity; innate immune response; signal transduction