Goat Coagulation factor VIII related antigen ELISA Kit | F8-Ag elisa kit

Goat Coagulation factor VIII related antigen ELISA Kit

Cat. Num. AAA747230

• Gene Names: F8; AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• Reactivity: Goat
• Synonyms: Coagulation factor VIII related antigen; Goat Coagulation factor VIII related antigen ELISA Kit; F8-Ag elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This F8-AG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat F8-AG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F8-Ag elisa kit

Principle of the assay: F8-AG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F8-AG antibody and an F8-AG-HRP conjugate. The assay sample and buffer are incubated together with F8-AG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F8-AG concentration since F8-AG from samples and F8-AG-HRP conjugate compete for the anti-F8-AG antibody binding site. Since the number of sites is limited, as more sites are occupied by F8-AG from the sample, fewer sites are left to bind F8-AG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F8-AG concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503647
• NCBI GeneID: 2157
• NCBI Accession #: NP_000123.1
• NCBI GenBank Nucleotide #: NM_000132.3
• Molecular Weight: 24,641 Da
• NCBI Official Full Name: coagulation factor VIII isoform a
• NCBI Official Synonym Full Names: coagulation factor VIII, procoagulant component
• NCBI Official Symbol: F8
• NCBI Official Synonym Symbols: AHF; F8B; F8C; HEMA; FVIII; DXS1253E
• NCBI Protein Information: coagulation factor VIII; factor VIII F8B; antihemophilic factor; coagulation factor VIIIc
• UniProt Protein Name: Coagulation factor VIII
• UniProt Gene Name: F8
• UniProt Synonym Gene Names: F8C; AHF
• UniProt Entry Name: FA8_HUMAN

NCBI Description

This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]

Uniprot Description

F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity; oxidoreductase activity

Biological Process: platelet activation; platelet degranulation; acute-phase response; proteolysis; blood coagulation; blood coagulation, intrinsic pathway

Disease: Hemophilia A; Factor Viii Deficiency