Rabbit anti-Human SPHK1 Polyclonal Antibody | anti-SPHK1 antibody

SPHK1, CT (R414) (SPHK1, SPHK, SPK, Sphingosine kinase 1) (APC)

Cat. Num. AAA6350531

• Gene Names: SPHK1; SPHK
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: SPHK1; Polyclonal Antibody; CT (R414) (SPHK1; SPHK; SPK; Sphingosine kinase 1) (APC); Sphingosine kinase 1; anti-SPHK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SPHK1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-438 amino acids from the C-terminal region of human SPHK1.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SPHK1 Antibody (C-term R414) in 293 cell line lysates (35ug/lane).SPHK1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SPHK1 Antibody (C-term R414) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.)

Flow Cytometry (FC/FACS)

(SPHK1 Antibody (C-term R414) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SPHK1 antibody

SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.

Product Categories/Family for anti-SPHK1 antibody

Antibodies; Protein Kinases

References

Hengst, J.A., et al. Arch. Biochem. Biophys. 494(1):23-31(2010). Jarman, K.E., et al. J. Biol. Chem. 285(1):483-492(2010). Gamble, J.R., et al. Am. J. Pathol. 175(5):2217-2225(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 74729085
• NCBI GeneID: 8877
• UniProt Accession #: Q8N632; Q9NYA1
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]