Rabbit ILP-2 Polyclonal Antibody | anti-BIRC8 antibody

ILP-2 Antibody

Cat. Num. AAA150864

• Gene Names: BIRC8; ILP2; ILP-2; hILP2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ILP-2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ILP-2; Polyclonal Antibody; ILP-2 Antibody; ILP2; hILP2; Baculoviral IAP repeat-containing protein 8; Inhibitor of apoptosis-like protein 2; IAP-like protein 2; baculoviral IAP repeat-containing 8; anti-BIRC8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ILP-2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 236

• Applicable Applications for anti-BIRC8 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ILP-2 antibody can be used for detection of ILP-2 by Western blot at 1 mug/mL. A band at approximately 33 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ILP-2 antibody was raised with against a synthetic peptide corresponding to amino acids near the amino terminus of human ILP-2.
• Buffer: ILP-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ILP-2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ILP-2 expression in human HepG2 (lane 1) and MOLT4 (lane 2) cell lysates with ILP-2 antibody at 1 μg/mL. )

Immunocytochemistry (IHC)

(Immunocytochemistry of ILP-2 in HepG2 cells with ILP-2 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ILP-2 in HepG2 cells with ILP-2 antibody at 20 μg/mL.)

Related Product Information for anti-BIRC8 antibody

ILP-2 Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2. ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues. ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.

NCBI and Uniprot Product Information

• NCBI GI #: 44680139
• NCBI GeneID: 112401
• NCBI Accession #: NP_203127
• NCBI GenBank Nucleotide #: NM_033341.4
• UniProt Accession #: Q96P09; Q6IPY1; Q96RW5
• Molecular Weight: 33 kDa
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 8
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 8
• NCBI Official Symbol: BIRC8
• NCBI Official Synonym Symbols: ILP2; ILP-2; hILP2
• NCBI Protein Information: baculoviral IAP repeat-containing protein 8; IAP-like protein 2; baculoviral IAP repeat-containing 8; inhibitor of apoptosis-like protein 2; testis-specific inhibitor of apoptosis
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 8
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC8
• UniProt Synonym Gene Names: ILP2; IAP-like protein 2; ILP-2
• UniProt Entry Name: BIRC8_HUMAN

Uniprot Description

BIRC8: Protects against apoptosis mediated by BAX. Belongs to the IAP family.

Protein type: Ubiquitin conjugating system; Apoptosis

Chromosomal Location of Human Ortholog: -

Cellular Component: spindle microtubule; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protease binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity

Biological Process: neuron apoptosis; protein ubiquitination; negative regulation of neuron apoptosis; negative regulation of apoptosis