Rabbit anti-Human Livin Polyclonal Antibody | anti-Livin antibody

Livin Antibody

Cat. Num. AAA9404563

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Livin; Polyclonal Antibody; Livin Antibody; KIAP; anti-Livin antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 280

• Applicable Applications for anti-Livin antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Livin antibody was raised with a synthetic peptide corresponding to amino acids 264 to 280 of. The short form and 281 to 298 of the long form of human Livin. This sequence is identical between a and b forms of the Livin proteins.
• Target Name: Livin
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Livin expression in human Raji cell lysate with Livin antibody at 0.5 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Livin in human small intestine tissue with Livin antibody at 5 ug/mL.)

Related Product Information for anti-Livin antibody

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-alpha and Livin-beta. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.

Product Categories/Family for anti-Livin antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 11545910
• NCBI GeneID: 79444
• NCBI Accession #: NP_071444
• NCBI GenBank Nucleotide #: NM_022161.3
• Molecular Weight: 30,866 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 7 isoform beta
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
• UniProt Entry Name: BIRC7_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]

Uniprot Description

BIRC7: Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. Binds to CASP9. Interaction with DIABLO/SMAC via the BIR domain disrupts binding to CASP9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with CASP3 and CASP7 via its BIR domain. Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. Belongs to the IAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: Golgi apparatus; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; ligase activity

Biological Process: regulation of signal transduction; protein ubiquitination; activation of JNK activity; negative regulation of apoptosis; regulation of cell proliferation