Rabbit IL7R / CD127 Polyclonal Antibody | anti-IL7R antibody

Rabbit Polyclonal to Mouse IL7R / CD127

Cat. Num. AAA242246

• Gene Names: IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: IL7R / CD127; Polyclonal Antibody; Rabbit Polyclonal to Mouse IL7R / CD127; Anti-IL7R / CD127 Antibody (C-Terminus) IHC-plus; IL7R; CD127; CD127 antigen; IL-7RA; IL-7 receptor subunit alpha; IL7RA; ILRA; Interleukin 7 receptor; CDW127; IL-7R subunit alpha; IL-7R-alpha; Mouse IL7R; anti-IL7R antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for mouse IL-7 receptor protein. A BLAST analysis was used to suggest cross-reactivity with IL-7 receptor protein from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 receptor protein from other sources has not been determined. Anti-IL-7 receptor alpha chain antibody is useful for researchers interested in Immunology Research.
• Purity/Purification: Affinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 459

• Applicable Applications for anti-IL7R antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:50000); IHC-P (5 ug/ml); WB (1:1000 - 1:10000); ; Usage: Immunohistochemistry: MBS242246 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242246 was determined to be 5 ug/ml.; ; Western Blot: Expect a band approximately 55 kD in size corresponding to IL-7 in the appropriate cell lysate or extract.; ; (optimal dilution to be determined by the researcher)
• Target Species: Mouse
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 receptor protein.
• Antigen Type: Synthetic peptide
• Immunogen Species: IL7R / CD127 antibody was raised against Human.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.

Immunohistochemistry (IHC)

(Anti-IL-7 Receptor antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Dot Blot of Rabbit anti-IL-7 Receptor antibody. Antigen: IL-7 Receptor and IL-7 Receptor pY449 forms of the immunizing peptide and THRB1 and THRB1 pY171 as controls. Load: 3.0, 0.3, 0.1, 0.05 ug as indicated. Primary antibody: IL-7 Receptor antibody at 1:400 for 45 min at 4°C. Secondary antibody: Dylight488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.)

Related Product Information for anti-IL7R antibody

Interleukin 7 Receptor (IL7R) is a receptor for interleukin 7 (IL7). The function of IL7R requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation.

Product Categories/Family for anti-IL7R antibody

Family: Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 28610151
• NCBI GeneID: 3575
• NCBI Accession #: NP_002176.2
• NCBI GenBank Nucleotide #: NM_002185.3
• UniProt Accession #: P16871; Q05CU8; Q6NSP4; Q6NWM0; Q6NWM1; Q6NWM2; Q6NWM3; Q6SV45; Q9UPC1; B2RCS6; B4DVT1
• NCBI Official Full Name: interleukin-7 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 7 receptor
• NCBI Official Symbol: IL7R
• NCBI Official Synonym Symbols: ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
• NCBI Protein Information: interleukin-7 receptor subunit alpha; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6
• UniProt Protein Name: Interleukin-7 receptor subunit alpha
• Protein Family: Interleukin-7 receptor
• UniProt Gene Name: IL7R
• UniProt Synonym Gene Names: IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA
• UniProt Entry Name: IL7RA_HUMAN