Western Blot (WB)
(Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) in 293 cell line lysates (35ug/lane). MLLT3(arrow) was detected using the purified Pab.)
Rabbit anti-Human AF9 Polyclonal Antibody | anti-AF9 antibody
AF9, CT (K486) (MLLT3, AF9, YEATS3, Protein AF-9, ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, YEATS domain-containing protein 3) (AP)
Gene Names
MLLT3; AF9; YEATS3
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
AF9; Polyclonal Antibody; CT (K486) (MLLT3; YEATS3; Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3) (AP); MLLT3; YEATS domain-containing protein 3; anti-AF9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-AF9 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
AF9 (MLLT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479~509 amino acids from the C-terminal region of human MLLT3.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) in 293 cell line lysates (35ug/lane). MLLT3(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of AF9 (MLLT3) Antibody (C-term K486) in 293 cell line lysates (35ug/lane). MLLT3(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-AF9 antibody
References
Iida, S., et al., Oncogene 8(11):3085-3092 (1993). Nakamura, T., et al., Proc. Natl. Acad. Sci. U.S.A. 90(10):4631-4635 (1993). Strissel, P. L., et al., Hum. Molec. Genet. 9: 1671-1679 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
568
NCBI Official Full Name
Protein AF-9
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
NCBI Official Symbol
MLLT3
NCBI Official Synonym Symbols
AF9; YEATS3
NCBI Protein Information
protein AF-9; YEATS domain-containing protein 3; ALL1-fused gene from chromosome 9 protein; myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3
UniProt Protein Name
Protein AF-9
UniProt Gene Name
MLLT3
UniProt Synonym Gene Names
AF9; YEATS3
UniProt Entry Name
AF9_HUMAN
Uniprot Description
MLLT3: A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
Protein type: Cell development/differentiation; Oncoprotein
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: transcription elongation factor complex; nucleus
Molecular Function: protein binding
Biological Process: anterior/posterior pattern formation; segment specification; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on AF9
Similar Products
Product Notes
The AF9 mllt3 (Catalog #AAA6272047) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AF9, CT (K486) (MLLT3, AF9, YEATS3, Protein AF-9, ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, YEATS domain-containing protein 3) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AF9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AF9 mllt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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