Western Blot (WB)
(Anti-IL5Ra rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDaObserved band size:44 kDa)
Rabbit anti-Mouse IL5RA Polyclonal Antibody | anti-IL5RA antibody
Anti-IL5RA Antibody, Rabbit Polyclonal
Gene Names
Il5ra; Il5r; CD125; CDw125
Reactivity
Mouse
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
IL5RA; Polyclonal Antibody; Anti-IL5RA Antibody; Rabbit Polyclonal; IL5RA (CD125) Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 5 receptor; alpha; Anti-CD125 Antibody; Anti-CDw125 Antibody; Anti-Il5r Antibody; anti-IL5RA antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse IL5RA (CD125)
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-IL5RA antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant Mouse IL5RA (CD125) Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse IL5RA (CD125) (rM IL5RA (CD125)). IL5RA (CD125) specific IgG was purified by Mouse IL5RA (CD125) affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-IL5Ra rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDaObserved band size:44 kDa)
Western Blot (WB)
(Anti-IL5Ra rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDaObserved band size:44 kDa)
Related Product Information for anti-IL5RA antibody
Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand-specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony-stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine that is particularly important in the development of asthma for the terminal differentiation, activation, and survival of committed eosinophil precursors.
Product Categories/Family for anti-IL5RA antibody
References
Isobe M, et al. (1992) Localization of the gene encoding the alpha subunit of human interleukin-5 receptor (IL5RA) to chromosome region 3p24-3p26. Genomics. 14(3): 755-8.Cheong HS, et al. (2005) Association analysis of interleukin 5 receptor alpha subunit (IL5RA) polymorphisms and asthma. J Hum Genet. 50(12): 628-34.Isobe M, et al. (1992) Localization of the gene encoding the alpha subunit of human interleukin-5 receptor (IL5RA) to chromosome region 3p24-3p26. Genomics. 14(3): 755-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,989 Da
NCBI Official Full Name
interleukin-5 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 5 receptor, alpha
NCBI Official Symbol
Il5ra
NCBI Official Synonym Symbols
Il5r; CD125; CDw125
NCBI Protein Information
interleukin-5 receptor subunit alpha
UniProt Protein Name
Interleukin-5 receptor subunit alpha
Protein Family
UniProt Gene Name
Il5ra
UniProt Synonym Gene Names
Il5r; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA
Uniprot Description
IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 6 E1|6 49.19 cM
Biological Process: inflammatory response to antigenic stimulus; regulation of interleukin-5 production
Research Articles on IL5RA
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Product Notes
The IL5RA il5ra (Catalog #AAA8112376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL5RA Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL5RA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the IL5RA il5ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL5RA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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