IL5Ra / CD125 recombinant protein

Recombinant Human IL5Ra / CD125 Protein (His tag)

Cat. Num. AAA2546288

• Gene Names: IL5RA; IL5R; CD125; CDw125; HSIL5R3
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: IL5Ra / CD125; Recombinant Human IL5Ra / CD125 Protein (His tag); CD125; CDw125; HSIL5R3; MIL5R; MGC26560; IL5Ra / CD125 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 420

• Application Notes: The recombinant human IL5Ra consists of 326 amino acids after removal of the signal peptide and has a predicted molecular mass of 37.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL5Ra is approximately 45-50 kDa due to glycosylation.
• Predicted N Terminal: Asp 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for IL5Ra / CD125 recombinant protein

Background: Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine which is particularly important in the development of asthma for the lerminal differentiation, activation and survival of committed cosinophil precursors.

Description: A DNA sequence encoding the human IL5Ra isoform 2 (Q01344-2) (Met 1-Arg 335) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 28559021
• NCBI GeneID: 3568
• NCBI Accession #: NP_000555.2
• NCBI GenBank Nucleotide #: NM_000564.4
• UniProt Accession #: Q01344; Q14633; Q15469; Q6ISX9; B3IU77; B4E2G0
• Molecular Weight: 23,737 Da
• NCBI Official Full Name: interleukin-5 receptor subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 5 receptor subunit alpha
• NCBI Official Symbol: IL5RA
• NCBI Official Synonym Symbols: IL5R; CD125; CDw125; HSIL5R3
• NCBI Protein Information: interleukin-5 receptor subunit alpha
• UniProt Protein Name: Interleukin-5 receptor subunit alpha
• UniProt Gene Name: IL5RA
• UniProt Synonym Gene Names: IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA

NCBI Description

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]

Uniprot Description

IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: extracellular space; plasma membrane

Molecular Function: interleukin-5 receptor activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: cell proliferation; MAPKKK cascade; signal transduction