Rabbit IL-7 Polyclonal Antibody | anti-IL7 antibody

RABBIT ANTI HUMAN INTERLEUKIN-7

Cat. Num. AAA221554

• Gene Names: IL7; IL-7
• Applications: ELISA, Functional Assay, Western Blot
• Synonyms: IL-7; Polyclonal Antibody; RABBIT ANTI HUMAN INTERLEUKIN-7; anti-IL7 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product, a Rabbit anti Human IL-7 antibody, is specific for human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7R? chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0 mg/ml after reconstitution (varies by lot)
• Sequence Length: 177

• Applicable Applications for anti-IL7 antibody: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Rabbit anti Human IL-7 (purified) may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with recombinant human IL-7 as the standard and Rabbit anti Human IL-7 (biotin conjugate) as the detection reagent.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0 ug/ml; Functional Assays: Minimum Dilution: 0.05; Maximum Dilution: 0.08ug/ml; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Immunogen: Recombinant human IL-7; Buffer Solution
• Antiserum Preparation: Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 6 months from date of reconstitution.

Related Product Information for anti-IL7 antibody

This product, a Rabbit anti Human IL-7 antibody, is specific for human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 4504677
• NCBI GeneID: 3574
• NCBI Accession #: NP_000871.1
• NCBI GenBank Nucleotide #: NM_000880.3
• UniProt Accession #: P13232; Q5FBY5; Q5FBY9; A0N0L3
• Molecular Weight: 7,687 Da
• NCBI Official Full Name: interleukin-7 isoform 1
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: IL7
• NCBI Official Synonym Symbols: IL-7
• NCBI Protein Information: interleukin-7
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: IL7
• UniProt Synonym Gene Names: IL-7
• UniProt Entry Name: IL7_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

Uniprot Description

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Apoptosis; Cell development/differentiation; Cell cycle regulation; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of organ growth; humoral immune response; T cell lineage commitment; organ morphogenesis; cell-cell signaling; positive regulation of T cell differentiation; homeostasis of number of cells within a tissue; regulation of gene expression; positive regulation of cell proliferation; negative regulation of catalytic activity; positive regulation of B cell proliferation; negative regulation of apoptosis; bone resorption