Rat IL-7 Monoclonal Antibody | anti-IL7 antibody

RAT ANTI HUMAN INTERLEUKIN-7

Cat. Num. AAA211284

• Gene Names: IL7; IL-7
• Applications: ELISA
• Synonyms: IL-7; Monoclonal Antibody; RAT ANTI HUMAN INTERLEUKIN-7; anti-IL7 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BVD10-40F6
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Approximate Protein Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 64

• Applicable Applications for anti-IL7 antibody: ELISA (EIA)
• Buffer Solution: Borate buffered saline
• Target Species: Human
• Preparation and Storage: Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Sandwich ELISA analysis of human interleukin-7 using Rat anti Human IL-7 as a capture reagent and biotinylated Rat anti Human IL-7 (MBS210291) as a detection reagent with recombinant human IL-7 (MBS232250) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A human serum sample is indicated (green))

Related Product Information for anti-IL7 antibody

Rat anti Human Interleukin-7 antibody, clone BVD10-40F6 recognizes human interleukin 7 (IL-7), also known as Lymphopoietin 1 or pre-B cell factor. IL-7 is a 177 amino acid haematopoietic growth factor stimulating primarily early B and T lymphocytes. IL- 7 is required for development of T-cells in the thymus (Plum et al. 1996) and absence of the IL-7 receptor alpha chain (CD127) leads to a severe combined immunodeficiency condition through the lack of T-cell production (Roifman et al. 2000). IL-7 is also important post-thymically for homeostasis of naive T-cell populations and generation and survival of CD4+ memory T-cells (Kondrack et al. 2003). IL-7 deficiency or deficient function is also noted in immunodeficiency diseases including rheumatoid arthritis (Leonard 2005), chronic colitis (Totsuka et al. 2007). multiple sclerosis through CD127 (Svejgaard 2008) and diabetes (Li et al. 2011). Rat anti Human Interleukin-7 antibody, clone BVD10-40F6 has been used sucessfully in the development of a sensitive ELISA assay as a capture reagent for human IL-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4504677
• NCBI GeneID: 3574
• NCBI Accession #: NP_000871.1
• NCBI GenBank Nucleotide #: NM_000880.3
• UniProt Accession #: P13232; Q5FBY5; Q5FBY9; A0N0L3
• Molecular Weight: 7,687 Da
• NCBI Official Full Name: interleukin-7 isoform 1
• NCBI Official Synonym Full Names: interleukin 7
• NCBI Official Symbol: IL7
• NCBI Official Synonym Symbols: IL-7
• NCBI Protein Information: interleukin-7
• UniProt Protein Name: Interleukin-7
• Protein Family: Interleukin
• UniProt Gene Name: IL7
• UniProt Synonym Gene Names: IL-7
• UniProt Entry Name: IL7_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

Uniprot Description

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell development/differentiation; Cytokine; Apoptosis; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q12-q13

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: positive regulation of organ growth; humoral immune response; T cell lineage commitment; organ morphogenesis; cell-cell signaling; positive regulation of T cell differentiation; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of B cell proliferation; bone resorption; negative regulation of apoptosis