Rabbit IkB Epsilon Polyclonal Antibody | anti-IkB Epsilon antibody

IkB Epsilon antibody

Cat. Num. AAA837380

• Gene Names: Nfkbie; IKBE; IkB-E; ikB-epsilon; NF-kappa-BIE; I-kappa-B-epsilon
• Applications: Immunohistochemistry, Western Blot
• Purity: IkB Epsilon antibody was purified by immunoaffinity chromatography.
• Synonyms: IkB Epsilon; Polyclonal Antibody; IkB Epsilon antibody; Polyclonal IkB Epsilon; Anti-IkB Epsilon; IkB-epsilon; Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor; IkB-E; IkappaBepsilon; IKBE; NFKBIE; NF-kappa-BIE; Phospho Ser22; NF-kappa-B inhibitor epsilon; I-kappa-B-epsilon; anti-IkB Epsilon antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: IkB Epsilon antibody was purified by immunoaffinity chromatography.
• Form/Format: Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 364

• Applicable Applications for anti-IkB Epsilon antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:1000
• Biological Significance: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Belongs to the NF-kappa-B inhibitor family. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.
• Cross-Reactivity: Human, Mouse
• Immunogen: IkB Epsilon antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 22 (I-E-S(p)-L-R) derived from Human IkB-E as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-epsilon (Phospho-Ser22) antibody (left) or the same antibody preincubated with with blocking peptide (right))

Western Blot (WB)

(Western Blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-epsilon (Phospho-Ser22) antibody)

Related Product Information for anti-IkB Epsilon antibody

Rabbit polyclonal IkB Epsilon antibody

Product Categories/Family for anti-IkB Epsilon antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 2739158
• NCBI GeneID: 18037
• UniProt Accession #: O54910; Q3U686; Q9CZZ9; Q9D7U3
• Molecular Weight: 39,200 Da
• NCBI Official Full Name: IkB epsilon
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon
• NCBI Official Symbol: Nfkbie
• NCBI Official Synonym Symbols: IKBE; IkB-E; ikB-epsilon; NF-kappa-BIE; I-kappa-B-epsilon
• NCBI Protein Information: NF-kappa-B inhibitor epsilon
• UniProt Protein Name: NF-kappa-B inhibitor epsilon
• UniProt Gene Name: Nfkbie
• UniProt Synonym Gene Names: Ikbe; NF-kappa-BIE; IkB-E; IkB-epsilon; IkappaBepsilon
• UniProt Entry Name: IKBE_MOUSE

Uniprot Description

IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.

Protein type: Inhibitor; DNA-binding

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: negative regulation of transcription, DNA-dependent; D-serine transport