Rabbit IkB-epsilon Polyclonal Antibody | anti-IkB antibody

IkB-epsilon (Phospho-S22) Polyclonal Antibody

Cat. Num. AAA3004676

• Gene Names: NFKBIE; IKBE
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IkB-epsilon; Polyclonal Antibody; IkB-epsilon (Phospho-S22) Polyclonal Antibody; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkappaBepsilon; NFKBIE; IKBE; IkB epsilon; IkBepsilon; anti-IkB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-IkB-epsilon (S22) polyclonal antibody detects endogenous levels of IkB-epsilon protein only when phosphorylated at Ser22
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 500

• Applicable Applications for anti-IkB antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human IkB-epsilon around the phosphorylation site of Serine 22.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-IkB-epsilon (S22) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-IkB antibody

IkB-epsilon (I-kappa-B-epsilon), also known as NFKBIE (NFkappaB inhibitor epsilon) or IKBE, is a 500 amino acid protein that belongs to the NFkappaB inhibitor family. Localizing to the cytoplasm, IkB-epsilon is highly expressed in spleen, testis and lung, with lower levels of expression found in kidney, pancreas, heart, placenta, brain, granulocytes and macrophages. IkB-epsilon inhibits c-Rel and NFkappaB subunits p50, p52 and p65 by forming a complex with them in the cytoplasm, preventing them from activating genes in the nucleus. IkB-epsilon undergoes serine phosphorylation, resulting in the protein being marked for destruction via the ubiquitination pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 71274109
• NCBI GeneID: 4794
• NCBI Accession #: NP_004547.2
• NCBI GenBank Nucleotide #: NP_004547.2
• UniProt Accession #: O00221
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: NF-kappa-B inhibitor epsilon
• NCBI Official Synonym Full Names: NFKB inhibitor epsilon
• NCBI Official Symbol: NFKBIE
• NCBI Official Synonym Symbols: IKBE
• NCBI Protein Information: NF-kappa-B inhibitor epsilon
• UniProt Protein Name: NF-kappa-B inhibitor epsilon
• UniProt Gene Name: NFKBIE
• UniProt Synonym Gene Names: IKBE; NF-kappa-BIE; IkB-E; IkB-epsilon
• UniProt Entry Name: IKBE_HUMAN

NCBI Description

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Uniprot Description

IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.

Protein type: Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: cytoplasmic sequestering of transcription factor; D-serine transport