Goat HOXA10 Polyclonal Antibody | anti-HOXA10 antibody

Goat Polyclonal to Human HOXA10

Cat. Num. AAA248400

• Gene Names: HOXA10; PL; HOX1; HOX1H; HOX1.8
• Reactivity: Bovine, Chimpanzee, Horse, Human, Mouse, Pig, Rabbit, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: HOXA10; Polyclonal Antibody; Goat Polyclonal to Human HOXA10; Anti-HOXA10 Antibody (aa152-165) IHC-plus; Homeo box A10; Homeobox protein Hox-A10; Homeobox protein Hox-1.8; Homeobox protein HOXA10; Homeobox protein 1H; Homeobox protein Hox-1H; HOX1; HOX1H; Homeobox A10; HOX1.8; Human HOXA10; anti-HOXA10 antibody

• Host: Goat
• Reactivity: Bovine, Chimpanzee, Horse, Human, Mouse, Pig, Rabbit, Rat
• Clonality: Polyclonal
• Specificity: Human HOXA10
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 410

• Applicable Applications for anti-HOXA10 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (5 ug/ml), WB (0.2 - 0.6 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 38kD band observed in Human Skeletal Muscle lysates (calculated MW of 42.4kD according to NP_061824.3. Recommended concentration: 0.2-0.6 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide PQATSCSFAQNIKE from an internal region of human HOXA10 (NP_061824.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Mouse, Rat, Ferret, Bovine, Elephant, Panda, Rabbit, Horse, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: HOXA10 antibody was raised against synthetic peptide PQATSCSFAQNIKE from an internal region of human HOXA10 (NP_061824.3). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Mouse, Rat, Ferret, Bovine, Elephant, Panda, Rabbit, Horse, Pig (100%); Monkey, Chicken, Platypus (93%).
• Antigen Modification: aa152-165
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-HOXA10 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HOXA10 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(HOXA10 antibody (0.2 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 182765442
• NCBI GeneID: 3206
• NCBI Accession #: NP_061824.3
• NCBI GenBank Nucleotide #: NM_018951.3
• UniProt Accession #: P31260; O43370; O43605; Q15949; Q504T1
• Molecular Weight: 11,452 Da
• NCBI Official Full Name: homeobox protein Hox-A10
• NCBI Official Synonym Full Names: homeobox A10
• NCBI Official Symbol: HOXA10
• NCBI Official Synonym Symbols: PL; HOX1; HOX1H; HOX1.8
• NCBI Protein Information: homeobox protein Hox-A10; homeo box A10; homeobox protein 1H; homeobox protein HOXA10; homeobox protein Hox-1H; homeobox protein Hox-1.8
• UniProt Protein Name: Homeobox protein Hox-A10
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA10
• UniProt Synonym Gene Names: HOX1H
• UniProt Entry Name: HXA10_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the downstream homeobox A9 (HOXA9) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

HOXA10: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5'-AA[AT]TTTTATTAC-3'. Belongs to the Abd-B homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; histone deacetylase binding

Biological Process: anterior/posterior pattern formation; transcription, DNA-dependent; single fertilization; multicellular organismal development; male gonad development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; uterus development; skeletal development; proximal/distal pattern formation; embryonic limb morphogenesis